Migration of myogenic cells from the somites to the fore-limb buds of developing mouse embryos

Developmental Dynamics : an Official Publication of the American Association of Anatomists
L Y SzeD Paulin

Abstract

In this study, we have isolated newly formed somites from the caudal regions of 8.5 day mouse embryos and transplanted them orthotopically into correspondingly staged hosts at the level of the prospective limb-forming region. The experimental embryos were then cultured intact for 32-36 hr. The donor somites used were pre-labelled with DiI, a fluorescent lipophilic dye, or were obtained from transgenic embryos that carried a 1 kb 5' regulatory sequence of the desmin gene linked to the gene encoding Escherichia coli beta-galactosidase. The transgene is specifically expressed in skeletal muscles (Li et al. [1993] Development 117:947-959). The aim of these experiments was to show definitively that the musculature of the mammalian limb is derived from the somites. The results demonstrated that DiI-labelled cells from the implanted somites were able to invade the proximal region of the fore-limb bud during the course of development. The use of transgenic somites as grafts confirmed that some of the somitic cells found in the limbs were myogenic cells. To determine whether the displacement of somitic cells is an active or passive process, somatopleure obtained from the prospective limb-forming regions of day 8.5 day embryos was implan...Continue Reading

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Citations

Feb 18, 2006·Theoretical Biology & Medical Modelling·Jason C Fisher, Lawrence Bodenstein
Jun 6, 2000·Current Biology : CB·B KnightA R Horwitz
Dec 6, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P Georgiades, P M Brickell
Jun 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S E WebbD A Ede
Jan 1, 2003·The Journal of Comparative Neurology·Randal P BabiukJohn J Greer
Dec 28, 2017·Genesis : the Journal of Genetics and Development·Clarissa C Pasiliao, Sevan Hopyan
Dec 2, 2011·Development·Laurent YvernogeauJosiane Fontaine-Perus

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