PMID: 9662709Jul 15, 1998Paper

Mild acid stress as a differentiation trigger in Trypanosoma brucei

Molecular and Biochemical Parasitology
S RolinE Pays

Abstract

In vitro differentiation of Trypanosoma brucei from the bloodstream to the procyclic form is efficiently induced by the combination of cold shock from 37 to 27 degrees C and the addition of citrate/cis-aconitate (CCA) to the incubation medium. Here it is reported that exposure of pleomorphic bloodstream trypanosomes to mild acidic conditions (pH 5.5 for 2 h at 37 degrees C) not only accelerated the process of morphological transformation from long slender and intermediate to short stumpy bloodstream forms but also allowed their subsequent differentiation into procyclic forms even in the absence of CCA. This process appeared to involve the glycosylphosphatidylinositol-specific phospholipase C (GPI-PLC), since null GPI-PLC mutants (PLC-) appeared to be largely refractory to acid stress-induced differentiation. However, an effective response was restored upon reintegration of the GPI-PLC gene in the genome (PLC+).

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 11, 1990·European Journal of Biochemistry·K ZiegelbauerP Overath
Mar 1, 1991·Molecular and Biochemical Parasitology·D ZilbersteinC L Jaffe
Jan 1, 1987·Annual Review of Microbiology·F R Opperdoes
Apr 1, 1985·British Medical Bulletin·K Vickerman
Jun 1, 1981·European Journal of Biochemistry·H P Voorheis, B R Martin
Jan 1, 1994·Annual Review of Microbiology·D Zilberstein, M Shapira

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Citations

Jun 8, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Paula MacGregor, Keith R Matthews
Oct 7, 2003·International Journal for Parasitology·Matthias LinigerIsabel Roditi
May 18, 2001·Journal of Insect Physiology·A H. KollienG A. Schaub
Oct 8, 1999·Molecular and Biochemical Parasitology·C M OchattG A Cross
Feb 29, 2000·Molecular and Biochemical Parasitology·N Van Der Heyden, R Docampo
May 1, 2012·Nature Reviews. Microbiology·Paula MacGregorKeith R Matthews
Nov 14, 2002·Molecular Biology of the Cell·Ingrid B MüllerErik Vassella
Oct 24, 2007·PLoS Pathogens·Paul M GrandgenettJohn E Donelson
Oct 16, 2015·International Journal for Parasitology·Sabine BachmaierDan Zilberstein
Nov 13, 2007·Current Opinion in Microbiology·Katelyn Fenn, Keith R Matthews
Apr 11, 2017·PLoS Pathogens·Henriette ZimmermannMarkus Engstler
Feb 23, 2011·Memórias do Instituto Oswaldo Cruz·Juliana C SchmidtMarco A Krieger
Mar 9, 2018·Molecular Biology of the Cell·Igor CestariKenneth Stuart
Sep 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benoît StijlemansStefan Magez
Jan 14, 2017·Frontiers in Microbiology·Alicia Ponte-Sucre
Feb 6, 2020·Trends in Parasitology·Balázs SzöőrKeith R Matthews
Mar 23, 2021·The Journal of Biological Chemistry·Alexander C HaindrichDoris Rentsch

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