Mild heat shock induces proliferation, alkaline phosphatase activity, and mineralization in human bone marrow stromal cells and Mg-63 cells in vitro

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
C Shui, A Scutt

Abstract

Bone formation has been shown to be stimulated by local diathermy in vivo; however, the mechanisms involved in this heat-induced osteogenesis are unclear. In this study, we investigated the direct effect of temperature on human bone marrow-derived stromal cells (BMSCs) and the human osteoblast-like, osteosarcoma-derived MG-63 cells in culture conditions. Both cell types were shown to tolerate the transient exposure to mild heat shock conditions (1 h at 39-41 degrees C), and long-term (96 h) exposure at 39 degrees C stimulated DNA synthesis in BMSC but caused growth arrest in MG-63 cells. Furthermore, 1-h exposure to higher temperatures (42.5-45 degrees C) or continuous 96-h exposure to 40 degrees C or 41 degrees C inhibited the proliferation of both BMSCs and MG63 cells. The level of alkaline phosphatase (ALP) in these cells linearly correlated with the increase in temperature, and the ALP expression, either at the basal level or in response to 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], was enhanced after a single 1-h exposure to 42.5 degrees C. In addition, continuous incubation at 39 degrees C or repeated transient exposure to 39/41 degrees C greatly enhanced the ability of BMSCs to form mineralizing nodules. The heat shock prot...Continue Reading

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Citations

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