Mild Heat Treatment Primes Human CD34(+) Cord Blood Cells for Migration Toward SDF-1α and Enhances Engraftment in an NSG Mouse Model

Stem Cells
Maegan L CapitanoHal E Broxmeyer

Abstract

Simple efforts are needed to enhance cord blood (CB) transplantation. We hypothesized that short-term exposure of CD34(+) CB cells to 39.5°C would enhance their response to stromal-derived factor-1 (SDF-1), by increasing lipid raft aggregation and CXCR4 expression, thus leading to enhanced engraftment. Mild hyperthermia (39.5°C) significantly increased the percent of CD34(+) CB that migrated toward SDF-1. This was associated with increased expression of CXCR4 on the cells. Mechanistically, mild heating increased the percent of CD34(+) cells with aggregated lipid rafts and enhanced colocalization of CXCR4 within lipid raft domains. Using methyl-β-cyclodextrin (MβCD), an agent that blocks lipid raft aggregation, it was determined that this enhancement in chemotaxis was dependent upon lipid raft aggregation. Colocalization of Rac1, a GTPase crucial for cell migration and adhesion, with CXCR4 to the lipid raft was essential for the effects of heat on chemotaxis, as determined with an inhibitor of Rac1 activation, NSC23766. Application-wise, mild heat treatment significantly increased the percent chimerism as well as homing and engraftment of CD34(+) CB cells in sublethally irradiated non-obese diabetic severe combined immunodeficie...Continue Reading

References

Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Q MaT A Springer
Feb 11, 2000·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·J R Ostberg, E A Repasky
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·F C YangD A Williams
Feb 28, 2002·Nature Cell Biology·Miguel Angel Del PozoMartin Alexander Schwartz
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dzung H Nguyen, Dennis Taub
Dec 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kent W ChristophersonHal E Broxmeyer
Aug 29, 2003·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·J R OstbergE A Repasky
Feb 7, 2004·Science·Jun-Lin Guan
Feb 7, 2004·Science·Miguel A del PozoMartin A Schwartz
Feb 26, 2004·The Journal of Cell Biology·Concepción Gómez-MoutónSantos Mañes
Aug 18, 2004·Science·Kent W ChristophersonHal E Broxmeyer
Nov 16, 2004·Biophysical Journal·Peter I KuzminFredric S Cohen
Jan 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Raffaella GhittoniCosima T Baldari
May 3, 2005·Cancer Immunology, Immunotherapy : CII·Julie R Ostberg, Elizabeth A Repasky
May 14, 2005·Blood·Tsvee LapidotOrit Kollet
Sep 13, 2007·The Journal of Biological Chemistry·Ana J García-SáezPetra Schwille
Jan 25, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Baris E DayancElizabeth A Repasky
Sep 28, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bhuvana KatkereJames R Drake
Aug 30, 2011·Journal of Leukocyte Biology·Thomas A MaceElizabeth A Repasky
Sep 20, 2011·The Journal of Clinical Investigation·Daniel T FisherSharon S Evans
Jan 13, 2012·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Thomas A MaceElizabeth A Repasky
Aug 21, 2012·Annals of the New York Academy of Sciences·Hal E Broxmeyer
May 16, 2013·Blood·Karen K BallenHal E Broxmeyer
Jul 12, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Balint CsobozLaszlo Vigh

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Citations

Apr 14, 2016·Blood Cells, Molecules & Diseases·Steven Messina-Graham, Hal Broxmeyer
May 24, 2016·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Hal E Broxmeyer
Dec 20, 2016·Bone Marrow Transplantation·A Dahlberg, F Milano
Mar 8, 2019·Stem Cells Translational Medicine·Hal E Broxmeyer
May 3, 2019·Current Opinion in Hematology·Xinxin Huang, Hal E Broxmeyer
May 28, 2019·Current Stem Cell Reports·Louis M Pelus, Hal E Broxmeyer
May 16, 2019·Bone Marrow Transplantation·Hector MayaniHal E Broxmeyer
Jul 14, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Julie R PerlinLeonard I Zon
Aug 23, 2018·Cell Research·Xinxin Huang, Hal E Broxmeyer
Jul 6, 2019·Current Stem Cell Reports·Xinxin HuangHal E Broxmeyer
Jun 12, 2020·Stem Cells·Jane L LiesveldOmar S Aljitawi
Mar 12, 2020·Experimental Cell Research·Vikas Chander, Gurudutta Gangenahalli

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