Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
Ping GongChun-Sheng Li

Abstract

Complement activation has been implicated in ischemia/reperfusion injury. This study aimed to determine whether mild hypothermia (HT) inhibits systemic and cerebral complement activation after resuscitation from cardiac arrest. Sixteen minipigs resuscitated from 8 minutes of untreated ventricular fibrillation were randomized into two groups: HT group (n=8), treated with HT (33°C) for 12 hours; and normothermia group (n=8), treated similarly as HT group except for cooling. Blood samples were collected at baseline and 0.5, 6, 12, and 24 hours after return of spontaneous circulation (ROSC). The brain cortex was harvested 24 hours after ROSC. Complement and pro-inflammatory markers were detected using enzyme-linked immunosorbent assay. Neurologic deficit scores were evaluated 24 hours after ROSC. C1q, Bb, mannose-binding lectin (MBL), C3b, C3a, C5a, interleukin-6, and tumor necrosis factor-α levels were significantly increased under normothermia within 24 hours after ROSC. However, these increases were significantly reduced by HT. Hypothermia decreased brain C1q, MBL, C3b, and C5a contents 24 hours after ROSC. Hypothermic pigs had a better neurologic outcome than normothermic pigs. In conclusion, complement is activated through cla...Continue Reading

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Citations

Aug 6, 2016·Journal of Neurotrauma·Dominic T SchombergDhanansayan Shanmuganayagam
Jun 10, 2018·Scandinavian Journal of Immunology·Simon Foged HaugaardAnne-Mette Hvas
Jul 19, 2019·Experimental and Therapeutic Medicine·Teiji OdaKen-Ichi Matsumoto
Dec 14, 2016·Brain : a Journal of Neurology·Anna StokowskaMarcela Pekna
Dec 12, 2018·Journal of Intensive Care Medicine·Christopher JouJignesh K Patel
Oct 13, 2020·Frontiers in Pediatrics·Cinzia AuritiVincenzo Maria Di Ciommo

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

SPSS

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