Mild traumatic brain injury induces apoptotic cell death in the cortex that is preceded by decreases in cellular Bcl-2 immunoreactivity

Neuroscience
R RaghupathiT K McIntosh

Abstract

Although mild traumatic brain injury is associated with behavioral dysfunction and histopathological alterations, few studies have assessed the temporal pattern of regional apoptosis following mild brain injury. Anesthetized rats were subjected to mild lateral fluid-percussion brain injury (1.1-1.3 atm), and brains were evaluated for the presence of in situ DNA fragmentation (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling, TUNEL) and morphologic characteristics of apoptotic cell death (nuclear and cytoplasmic condensation, presence of apoptotic bodies). Significant numbers of apoptotic TUNEL(+) cells were observed in the injured parietal cortex and underlying white matter up to 72 h post-injury (P<0.05 compared to sham-injured-injured), with maximal numbers present at 24 h. Apoptosis was confirmed by the presence of 180-200 bp nuclear DNA fragments in tissue homogenates. The appearance of apoptotic TUNEL(+) cells in the injured cortex was preceded by a marked decrease in immunoreactivity for the anti-cell death protein, Bcl-2, as early as 2 h post-injury. This decrease in cellular Bcl-2 staining was not accompanied by a concomitant loss of staining for the pro-cell death Bax protein, suggesting tha...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Sep 1, 1990·Neurosurgery·D H WilliamsH M Eisenberg
Dec 1, 1988·The Journal of Trauma·J F Kraus, P Nourjah
Sep 1, 1995·Stroke; a Journal of Cerebral Circulation·M D LinnikH J Federoff
Feb 1, 1995·Journal of Neurotrauma·N AiharaL J Noble
Aug 1, 1994·Journal of Neurotrauma·T A Gennarelli
Jan 1, 1995·Brain Injury : [BI]·N I BohnenG Wijnen
Dec 31, 1993·Behavioural Brain Research·R J HammB R Pike
Jun 1, 1993·Annals of Emergency Medicine·J T Povlishock
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L T ZhongD E Bredesen
Mar 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C DuD W Choi
Aug 1, 1996·The Journal of Nervous and Mental Disease·M KleinJ Jolles
May 1, 1996·Journal of Neurotrauma·K FukudaL J Noble
Jul 1, 1996·Journal of Neuropathology and Experimental Neurology·K E SaatmanT K McIntosh
Jul 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M S LawrenceG K Steinberg
Mar 14, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M EndresM A Moskowitz
Nov 11, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R RaghupathiT K McIntosh
Jan 1, 1999·Journal of Neurotrauma·K E SaatmanT K McIntosh
Jan 23, 1999·Oncogene·J C Reed
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·U ScherbelT K McIntosh
Jul 21, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M NakamuraT K McIntosh
Jan 13, 2000·Trends in Neurosciences·K K Wang

❮ Previous
Next ❯

Citations

Sep 12, 2006·Intensive Care Medicine·Maria Angeles BallesterosEduardo Miñambres
Mar 28, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Tohru ShigaNagara Tamaki
Mar 1, 2012·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Kathleen M SchochKathryn E Saatman
Jan 25, 2005·Journal of Neurotrauma·Hilaire J ThompsonTracy K McIntosh
Aug 25, 2006·Journal of Neurotrauma·Tomer BegazJeffrey J Bazarian
Aug 25, 2006·Journal of Neurotrauma·Kathryn E SaatmanRamesh Raghupathi
Mar 28, 2008·Journal of Neurotrauma·Eduardo MiñambresMarcos López-Hoyos
Aug 12, 2010·Journal of Neurotrauma·Ken-ichiro NakaoHiroyoshi Koyama
Jan 5, 2013·Journal of Neurotrauma·Douglas S DewittClaudia S Robertson
Apr 5, 2011·Therapeutic Hypothermia and Temperature Management·George LotockiHelen M Bramlett
Apr 28, 2006·Current Opinion in Psychiatry·Grant L Iverson
Sep 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mary F DallmanSotara Manalo
Sep 28, 2013·Journal of Neurotrauma·Meghan O BlayaW Dalton Dietrich
Sep 19, 2014·Neurosurgery·Anthony L PetragliaJulian E Bailes
Apr 22, 2005·Intensive Care Medicine·Eduardo MiñambresMarcos López-Hoyos
Dec 25, 2010·Neurochemical Research·Liu JiaWang Ting-Hua
Jan 1, 2016·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Marcel Levy NogueiraLaurent Schwartz
Dec 1, 2009·Neuroscience Research·Alok KumarG Nagesh Babu
Jun 15, 2004·Brain Pathology·Ramesh Raghupathi
Jun 15, 2004·Brain Pathology·Jose A PinedaRonald L Hayes
Jan 24, 2007·Journal of Neuroscience Research·David TweedieNigel H Greig
Oct 7, 2015·Frontiers in Oncology·Marcel Levy NogueiraLaurent Schwartz
Nov 3, 2005·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Li-Qin ChenFeng-Yan Sun
May 24, 2014·Expert Review of Neurotherapeutics·Adrián González-LópezKonrad Talbot
Dec 31, 2010·Journal of Neurotrauma·Ahmed Mohammed SulaimanWilliam L Maxwell
Jul 4, 2007·Journal of Neuroinflammation·Anthony J WilliamsFrank C Tortella

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Alzheimer's Disease: Tau & TDP-43

Alzheimer's disease is a neurodegenerative disease. This feed focuses on the underlying role of tau proteins and TAR DNA-binding protein 43, as well as other genetic factors, in Alzheimer's disease.