Milk-Derived Tripeptides IPP (Ile-Pro-Pro) and VPP (Val-Pro-Pro) Enhance Insulin Sensitivity and Prevent Insulin Resistance in 3T3-F442A Preadipocytes

Journal of Agricultural and Food Chemistry
Subhadeep ChakrabartiJianping Wu

Abstract

There is great interest in developing naturally derived compounds, especially bioactive peptides with potential insulin sensitizing effects and/or preventing insulin resistance. Previously, we showed adipogenic and insulin mimetic actions of IPP (Ile-Pro-Pro) and VPP (Val-Pro-Pro), the milk-derived tripeptides on cultured preadipocytes, in addition to their previously characterized antihypertensive and anti-inflammatory functions. However, the effect of these peptides on insulin signaling is not known. Therefore, we examined IPP and VPP effects on insulin signaling in preadipocytes, a well-established model for studying insulin signaling. Our results suggested both peptides enhanced insulin signaling and contributed toward the prevention of insulin resistance in the presence of tumor necrosis factor (TNF). Inhibition of inflammatory mediator NF-kB under TNF stimulation was a likely contributor to the prevention of insulin resistance. VPP further enhanced the expression of glucose transporter 4 (GLUT4) in adipocytes and restored glucose uptake in TNF-treated adipocytes. Our data suggested the potential of these peptides in the management of conditions associated with impairments in insulin signaling.

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Citations

Nov 7, 2019·Critical Reviews in Food Science and Nutrition·Abdul Majid, Poornima Priyadarshini C G
Jun 20, 2019·Protein and Peptide Letters·Sulochana Priya
Jul 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Shanshan LiJianping Wu
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Kong Fei ChaiWei Ning Chen
Mar 12, 2020·Journal of Agricultural and Food Chemistry·Fidel ToldráLeticia Mora

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