Milk glucosidase activity enables suckled pup starch digestion

Molecular and Cellular Pediatrics
Buford L NicholsRoberto Quezada-Calvillo

Abstract

ᅟ: Starch requires six enzymes for digestion to free glucose: two amylases (salivary and pancreatic) and four mucosal maltase activities; sucrase-isomaltase and maltase-glucoamylase. All are deficient in suckling rodents. The objective of this study is to test (13)C-starch digestion before weaning by measuring enrichment of blood (13)C-glucose in maltase-glucoamylase-null and wild-type mice. Maltase-glucoamylase gene was ablated at the N-terminal. Dams were fed low (13)C-diet and litters kept on low (13)C-diet. Pups were weaned at 21 days. Digestion was tested at 13 and 25 days by intragastric feeding of amylase predigested (13)C-α-limit dextrins. Blood (13)C-glucose enrichment was measured by gas chromatography combustion isotope ratio mass spectrometry (GCRMS) using penta-acetate derivatives. Four hours after feeding, blood (13)C-glucose was enriched by 26 × 10(3) in null and 18 × 10(3) in wild-type mice at 13 days and 0.3 × 10(3) and 0.2 × 103 at 25 days (vs. fasting p = 0.045 and p = 0.045). By jejunal enzyme assay, immunohistochemistry, or Western blots, there was no maltase activity or brush border staining with maltase-glucoamylase antibodies at 13 days, but these were fully developed in the wild-type mice by 25 days. In...Continue Reading

References

Dec 1, 1989·The Journal of Pediatrics·J E Stuff, B L Nichols
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Feb 13, 2001·The Journal of Nutrition·S Fomon
Jun 19, 2003·The American Journal of Clinical Nutrition·Bo Lönnerdal
Jun 23, 2007·The Journal of Nutrition·Roberto Quezada-CalvilloBuford L Nichols
Jun 23, 2009·Journal of Pediatric Gastroenterology and Nutrition·Sonia SchiessUNKNOWN European Childhood Obesity Project
Nov 1, 2012·Journal of Pediatric Gastroenterology and Nutrition·Amy Hui-Mei LinBuford L Nichols
Mar 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Florent UbelmannSylvie Robine
Jul 11, 2013·Journal of Pediatric Gastroenterology and Nutrition·Maricela Diaz-SotomayorBuford L Nichols
Jun 16, 2015·The Journal of Pediatrics·Brennan VailKen K Ong

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Citations

May 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sunil K SukumaranRobert F Margolskee
Aug 16, 2016·Molecular and Cellular Pediatrics·Hassan Y NaimBuford Nichols

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