Milk progesterone enzyme-linked immunosorbent assay as a tool to investigate ovarian cyclicity of water buffaloes in relation to body condition score and milk production.

Acta Veterinaria Scandinavica
Turgish A BanuJalal U Ahmed

Abstract

Application of assisted reproductive technologies in buffaloes is limited to some extent by farmers' inability to detect oestrus because of its poor expression. The present study aimed at investigating reliability of a milk progesterone enzyme-linked immunosorbent assay (ELISA) to assess the ovarian cyclicity during post partum, oestrus and post-breeding periods in water buffaloes. Progesterone concentrations were measured by an ELISA in milk of 23 postpartum buffaloes in relation to oestrus, pregnancy, body condition score (BCS) and milk production. Two milk samples were taken at 10 days intervals, every month starting from day 30 and continued to day 150 post partum. BCS and milk production were recorded during sample collection. Milk samples from bred buffaloes were collected at Day 0 (day of breeding), Days 10-12 and Days 22-24. Defatted milk was preserved at -80°C until analysis. Pregnancy was confirmed by palpation per rectum on Days 70-90. Seventeen buffaloes had 47 ovulatory cycles, one to four in each, 13 were detected in oestrus once (28 % oestrus detection rate). Progesterone concentration ≥1 ng/ml in one of the two 10-day-interval milk samples reflected ovulation and corpus luteum formation. The intervals between ca...Continue Reading

References

Nov 1, 1990·The British Veterinary Journal·M Gupta, B S Prakash
Nov 1, 1987·The Journal of Dairy Research·R CapparelliA Bordi
Aug 1, 1993·Tropical Animal Health and Production·M Kamboj, B S Prakash
Jan 5, 2002·Theriogenology·M C WiltbankR Sartori
Apr 5, 2007·Tropical Animal Health and Production·M ShamsuddinD Galloway
Sep 28, 2010·Animal Reproduction Science·B M A O Perera

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Citations

Jun 9, 2015·General and Comparative Endocrinology·Shohei KobayashiGen Watanabe
Aug 20, 2019·Journal of Dairy Science·M T HarperA N Hristov

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
Enzyme linked immunosorbent assay

Software Mentioned

MS Excel
Excel

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