Milk protein synthesis is regulated by T1R1/T1R3, a G protein-coupled taste receptor, through the mTOR pathway in the mouse mammary gland

Molecular Nutrition & Food Research
JunQiang LiuChunLei Zhang

Abstract

Understanding the regulatory mechanism of milk protein synthesis is important to develop strategies to improve milk protein and enhance lactation performance. The mammalian target of rapamycin (mTOR) pathway is a crucial modulator of protein synthesis. In this study, we want to investigate if T1R1/T1R3 can regulate milk protein synthesis and mediate the mTOR pathway in the mice mammary gland in vivo. T1R1 knockout mice, WT mice, and mammary explants were used. The weigh-suckle-weigh method was used to quantify the milk yield. The expression level of β-casein and AA transporter mRNA were analyzed by qPCR. Western blot was used to analyze protein abundance of members of the mTOR pathway. As expected, the knockout of T1R1 not only reduced the total milk yield in the mice mammary glands, but also repressed β-casein synthesis. Additionally, the phosphorylation of 4EBP1 and S6K was significantly decreased in T1R1 knockout mice. The T1R1 knockout also increased the protein abundance of the AA transporter SLC3A2 and mRNA expression of SLC7A5/SLC3A2 and SLC1A5. Activation of the mTOR pathway was repressed by inhibition of T1R3 or T1R1 knockout in mammary gland explants. T1R1/T1R3 modulates the mTOR pathway to regulate milk protein synth...Continue Reading

References

Mar 10, 2001·Pediatric Clinics of North America·M F Picciano
Mar 15, 2002·Nature·Greg NelsonCharles S Zuker
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiaodong LiElliot Adler
Jul 19, 2003·Science·Sami DamakRobert F Margolskee
Nov 26, 2003·Cell·Grace Q ZhaoCharles S Zuker
Jan 26, 2005·The Journal of Biological Chemistry·Peihua JiangMarianna Max
Jan 21, 2006·Journal of Animal Science·J M McDonald, M K Nielsen
Feb 14, 2006·The American Journal of Clinical Nutrition·Scot R Kimball, Leonard S Jefferson
Mar 30, 2006·The Journal of Dairy Research·Henri Rulquin, Pawel M Pisulewski
Sep 23, 2006·Physiology·Xuemin Wang, Christopher G Proud
May 15, 2007·The Journal of Physiology·Oliver J MaceGeorge L Kellett
Sep 4, 2008·American Journal of Physiology. Endocrinology and Metabolism·Joseph AvruchNing Dai
Apr 3, 2009·Nature Reviews. Molecular Cell Biology·Xiaoju Max Ma, John Blenis
Jul 10, 2009·Frontiers in Integrative Neuroscience·Xueying RenIvan E de Araujo
Dec 16, 2010·Nature Reviews. Molecular Cell Biology·Roberto ZoncuDavid M Sabatini
Oct 4, 2011·Current Opinion in Cell Biology·Eva Dazert, Michael N Hall
Dec 21, 2011·The Journal of Physiology·Keiko YasumatsuYuzo Ninomiya
Sep 11, 2012·Molecular Cell·Eric M WausonMelanie H Cobb
Oct 4, 2013·Journal of Amino Acids·Angelos K SikalidisMartha H Stipanuk
Sep 10, 2014·Amino Acids·Rodrigo ManjarinNathalie L Trottier
May 23, 2015·The Journal of Endocrinology·Kunihisa HamanoItaru Kojima
Sep 18, 2015·The Journal of Biological Chemistry·Laura A Stransky, Michael Forgac
Oct 10, 2015·Science·Rachel L WolfsonDavid M Sabatini
Mar 15, 2016·Cell·Lynne ChantranupongDavid M Sabatini
Apr 15, 2016·Cell Metabolism·Deborah C I GoberdhanAdrian L Harris

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Citations

Jun 14, 2020·Animals : an Open Access Journal From MDPI·Shuangming YueBai Xue
Sep 21, 2018·Frontiers in Cell and Developmental Biology·Mariafrancesca ScaliseCesare Indiveri
Mar 11, 2020·Frontiers in Cell and Developmental Biology·Hongmei ZhuMingxing Ding

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