Millimeter-Deep Detection of Single Shortwave-Infrared-Emitting Polymer Dots through Turbid Media

Nano Letters
Hubert PiwońskiSatoshi Habuchi

Abstract

Fluorescence imaging at longer wavelengths, especially in the shortwave-infrared (SWIR: 1000-1700 nm) region, leads to a substantial decrease in light attenuation, scattering, and background autofluorescence, thereby enabling enhanced penetration into biological tissues. The limited selection of fluorescent probes is a major bottleneck in SWIR fluorescence imaging. Here, we develop SWIR-emitting nanoparticles composed of donor-acceptor-type conjugated polymers. The bright SWIR fluorescence of the polymer dots (primarily attributable to their large absorption cross-section and high fluorescence saturation intensity (as high as 113 kW·cm-2)) enables the unprecedented detection of single particles as small as 14 nm through millimeter-thick turbid media. Unlike most SWIR-emitting nanomaterials, which have an excited-state lifetime in the range of microseconds to milliseconds, our polymer dots exhibit a subnanosecond excited-state lifetime. These characteristics enable us to demonstrate new time-gated single-particle imaging with a high signal-to-background ratio. These findings expand the range of potential applications of single-particle deep-tissue imaging.

References

Sep 29, 2009·ACS Nano·Iwan MoreelsZeger Hens
Nov 10, 2009·Nature Nanotechnology·Andrew M SmithShuming Nie
Mar 8, 2011·Angewandte Chemie·Changfeng WuDaniel T Chiu
Nov 15, 2011·Biomaterials·Nan ChenChunhai Fan
Nov 20, 2012·Nature Medicine·Guosong HongHongjie Dai
Jul 23, 2013·Nature Communications·D J NaczynskiP V Moghe
Dec 11, 2014·Journal of the American Chemical Society·I-Che WuDaniel T Chiu
Mar 25, 2015·Journal of Biomedical Optics·Robert H WilsonAnthony J Durkin
Apr 14, 2015·Inhalation Toxicology·Katsuhide FujitaKazumasa Honda
Nov 26, 2015·Nature Materials·Alexander L AntarisHongjie Dai
Sep 1, 2014·Nature Photonics·Guosong HongHongjie Dai
Aug 15, 2017·Angewandte Chemie·Emily D CoscoEllen M Sletten
Sep 5, 2017·Reports on Progress in Physics·Helen MillerMark C Leake
Nov 2, 2017·Nature·Martin J Schnermann
Nov 10, 2017·Nature Biomedical Engineering·Oliver T BrunsMoungi G Bawendi
Apr 8, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jessica A CarrOliver T Bruns
Jul 30, 2019·Journal of the American Chemical Society·Wei ChenEllen M Sletten
Dec 31, 2019·Advanced Functional Materials·Hao WanHongjie Dai
Jan 14, 2020·Journal of Pharmaceutical Analysis·Debashish MohantaNilanjan Das
May 25, 2016·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Eva HemmerFiorenzo Vetrone

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