Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron

IUCrJ
Sylvain AumonierAntoine Royant

Abstract

The recent development of serial crystallography has popularized time-resolved crystallography as a technique to determine the structure of protein-reaction intermediate states. However, most approaches rely on the availability of thousands to millions of microcrystals. A method is reported here, using monochromatic synchrotron radiation, for the room-temperature collection, processing and merging of X-ray oscillation diffraction data from <100 samples in order to observe the build up of a photoreaction intermediate species. Using this method, we monitored with a time resolution of 63 ms how the population of a blue-light photoreceptor domain in a crystal progressively photoconverts from the dark to the light state. The series of resulting snapshots allows us to visualize in detail the gradual rearrangement of both the protein and chromophore during this process.

References

Nov 4, 2000·Nature Structural Biology·J HajduC M Wilmot
Jul 10, 2001·The Journal of Biological Chemistry·T E SwartzR A Bogomolni
Mar 26, 2003·Biochemistry·John T M KennisRienk van Grondelle
Jun 21, 2003·Science·Friedrich SchottePhilip A Anfinrud
Sep 13, 2003·Science·Shannon M HarperKevin H Gardner
Sep 1, 2005·Current Opinion in Structural Biology·Dominique Bourgeois, Antoine Royant
Feb 24, 2006·Journal of Synchrotron Radiation·Ch BroennimannA Wagner
Oct 28, 2006·Annual Review of Plant Biology·John M Christie
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Feb 5, 2011·Nature·Henry N ChapmanJohn C H Spence
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Philip R Evans
Jan 11, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Matthew J WheelerMatthew W Bowler
May 26, 2012·Science·P Andrew Karplus, Kay Diederichs
Nov 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Friedrich SchottePhilip A Anfinrud
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Philip R Evans, Garib N Murshudov
Dec 10, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Andrei S Halavaty, Keith Moffat
Jan 24, 2015·Acta Crystallographica. Section D, Biological Crystallography·David von StettenAntoine Royant
Sep 8, 2016·Acta Crystallographica. Section D, Structural Biology·Arnau CasanasMeitian Wang
Dec 9, 2017·Journal of Applied Crystallography·Gianluca SantoniAlexander Popov
Jan 23, 2018·Journal of Physics D: Applied Physics·Vukica Šrajer, Marius Schmidt
Mar 2, 2019·Acta Crystallographica. Section D, Structural Biology·Marie Luise Grünbein, Gabriela Nass Kovacs
Jul 19, 2019·IUCrJ·Guillaume GotthardAntoine Royant
Sep 19, 2019·Nature Methods·Pedram MehrabiR J Dwayne Miller
May 10, 2020·Journal of Synchrotron Radiation·David von StettenChristoph Mueller-Dieckmann

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Citations

Feb 5, 2021·Emerging Topics in Life Sciences·Jason E SchafferJoseph M Jez
Aug 28, 2021·Science·Gisela Brändén, Richard Neutze

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Methods Mentioned

BETA
X-ray
size-exclusion chromatography

Software Mentioned

Coot
Phenix
AIMLESS
POINTLESS
ccCluster
XDS

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