Millisecond-timescale, genetically targeted optical control of neural activity

Nature Neuroscience
Edward S BoydenKarl Deisseroth

Abstract

Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons. We demonstrate reliable, millisecond-timescale control of neuronal spiking, as well as control of excitatory and inhibitory synaptic transmission. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers.

References

Jun 9, 1995·Science·Z F Mainen, T J Sejnowski
Oct 1, 1994·Journal of Neuroscience Methods·L C Katz, M B Dalva
Oct 10, 1998·Journal of Virology·T DullL Naldini
Mar 23, 1999·Journal of Neurophysiology·D L PettitW C Hall
Jun 22, 2000·Nature Neuroscience·J L Dantzker, E M Callaway
Jan 10, 2002·Neuron·Boris V ZemelmanGero Miesenböck
May 2, 2002·Journal of Neurobiology·Hajime HiraseRafael Yuste
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Oleg A SineshchekovJohn L Spudich
Jun 14, 2002·Neuron·Michael S A GrazianoTirin Moore
Jun 29, 2002·Science·Georg NagelPeter Hegemann
Jan 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Boris V ZemelmanGero Miesenbock
Jan 24, 2003·Nature·Tirin Moore, Katherine M Armstrong
Feb 5, 2003·Biochemical and Biophysical Research Communications·Takeshi SuzukiTetsuo Takahashi
Jul 15, 2003·Nature Neuroscience·Jochen DitterichMichael N Shadlen
May 1, 1961·Journal of Neurophysiology·E R KANDELF J BRINLEY
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
May 26, 2004·Neuron·Karl DeisserothRobert C Malenka
Jun 1, 2004·Nature·Frédéric Pouille, Massimo Scanziani
Nov 24, 2004·Nature Neuroscience·Matthew BanghartRichard H Kramer
Feb 25, 2005·Nature·Yumiko YoshimuraEdward M Callaway

❮ Previous
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Citations

Jul 26, 2011·Cellular and Molecular Life Sciences : CMLS·Huisheng Liu, Su-Chun Zhang
Sep 20, 2012·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Juuso NissiläSeppo Saarela
Apr 12, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·José-Alain SahelSaddek Mohand-Said
Feb 19, 2013·Pflügers Archiv : European journal of physiology·Taofei Yin, Yi I Wu
Dec 21, 2011·Molecular Genetics and Genomics : MGG·Martin L Rein, Jan M Deussing
May 23, 2013·Cell and Tissue Research·Christian T WentzWolfgang Kelsch
Sep 15, 2012·Neurogenetics·Jessica M Kelly, Matt T Bianchi
Apr 10, 2012·Annals of Biomedical Engineering·Shan GaoMartin L Yarmush
Dec 31, 2010·Biomedical Microdevices·Steven Y LeighEmilia Entcheva
Dec 22, 2010·Journal of Computational Neuroscience·Sachin S TalathiPramod P Khargonekar
Nov 28, 2012·Journal of Computational Neuroscience·Nir GrossmanKonstantin Nikolic
Jul 26, 2008·Brain Cell Biology·Philipp SchoenenbergerThomas G Oertner
Aug 6, 2008·Brain Cell Biology·B J BakerT Knöpfel
Aug 5, 2008·Brain Cell Biology·Viviana GradinaruKarl Deisseroth
Oct 22, 2008·Brain Cell Biology·Shengli ZhaoGuoping Feng
Feb 19, 2011·Neuroinformatics·Rodney J Douglas, Kevan A C Martin
Oct 16, 2012·Molecular Neurobiology·Aron KosArmaz Aschrafi
Jan 22, 2010·Journal of Genetics·Hiroshi TomitaMakoto Tamai
Jul 27, 2012·Neuroscience Bulletin·Quanhui ChenZhian Hu
Mar 4, 2011·The Journal of Microbiology·Akira KawanabeHideki Kandori
Mar 5, 2013·Behavioural Brain Research·Aldis P Weible
Mar 5, 2013·European Journal of Pharmacology·Philip A BarishWilliam O Ogle
May 15, 2013·Neuropharmacology·Satoshi Ikemoto, Antonello Bonci
Apr 30, 2013·Trends in Genetics : TIG·Annelies Gerits, Wim Vanduffel
Nov 7, 2008·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Grace Stutzmann
Aug 17, 2012·ACS Chemical Neuroscience·Mikhail G ShapiroHenry A Lester
Aug 17, 2012·ACS Chemical Neuroscience·Xue Han
Aug 17, 2012·ACS Chemical Neuroscience·Alan Jasanoff
Feb 20, 2013·ACS Chemical Neuroscience·Joyce G RohanRobert H Chow
May 9, 2009·Journal of the American Chemical Society·Ionela RaduJoachim Heberle
Mar 22, 2013·ACS Nano·A Paul AlivisatosXiaowei Zhuang
May 7, 2010·Nature·Lizzie Buchen
Jun 11, 2010·Nature·David A Leopold
Oct 29, 2013·Acta Pharmacologica Sinica·Yu-feng XieJohn F Macdonald
Dec 12, 2012·Cell Death & Disease·X SunY Zhang
Nov 2, 2013·Cell Death & Disease·F YangL-P Wang
Oct 14, 2011·Gene Therapy·V BusskampB Roska
Apr 21, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·M Mehdi DoroudchiAlan Horsager
Apr 28, 2009·Nature·Jessica A CardinChristopher I Moore
Oct 16, 2009·Nature·Benjamin R Arenkiel, Michael D Ehlers
Apr 24, 2010·Nature·Hillel Adesnik, Massimo Scanziani

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