Miltenberger blood group typing by real-time polymerase chain reaction (qPCR) melting curve analysis in Thai population

Transfusion Medicine
A VongsakulyanonT Srikhirin

Abstract

To develop reliable and convenient methods for Miltenberger (Mi(a) ) blood group typing. To apply real-time polymerase chain reaction (qPCR) melting curve analysis to Mi(a) blood group typing. The Mi(a) blood group is the collective set of glycophorin hybrids in the MNS blood group system. Mi(a+) blood is common among East Asians and is also found in the Thai population. Incompatible Mi(a) blood transfusions pose the risk of life-threatening haemolysis; therefore, Mi(a) blood group typing is necessary in ethnicities where the Mi(a) blood group is prevalent. One hundred and forty-three blood samples from Thai blood donors were used in the study. The samples included 50 Mi(a+) samples and 93 Mi(a-) samples, which were defined by serology. The samples were typed by Mi(a) typing qPCR, and 50 Mi(a+) samples were sequenced to identify the Mi(a) subtypes. Mi(a) subtyping qPCR was performed to define GP.Mur. Both Mi(a) typing and Mi(a) subtyping were tested on a conventional PCR platform. The results of Mi(a) typing qPCR were all concordant with serology. Sequencing of the 50 Mi(a+) samples revealed 47 GP.Mur samples and 3 GP.Hop or Bun samples. Mi(a) subtyping qPCR was the supplementary test used to further define GP.Mur from other Mi...Continue Reading

Citations

Jan 1, 1975·Vox Sanguinis·D Chandanyingyong, S Pejrachandra
Jul 1, 1992·Transfusion Medicine Reviews·P TippettD J Anstee
Mar 1, 1966·Vox Sanguinis·T E Cleghorn
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Nov 16, 2011·Nucleic Acids Research·Santosh Kumar PatnaikOlga O Blumenfeld

Related Concepts

Real-Time Polymerase Chain Reaction
Blood Grouping and Crossmatching
Study
Thais (Population Group)
GYPA
Kinetic Polymerase Chain Reaction
Glycophorin E
Nucleic Acid Sequencing
Donor Person
Ethnic Group

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