Mimetic ligand-based affinity purification of immune complexes and immunoconjugates

Journal of Chromatography. B, Biomedical Sciences and Applications
D XuM R Suresh

Abstract

We developed a simple purification method to purify alkaline phosphatase/anti-alkaline phosphatase IgG as immune complexes using mimetic affinity chromatography wherein the antibody was either a monospecific antibody, a bispecific antibody or a commercial polyclonal IgG conjugated with alkaline phosphatase (AP-IgG) covalently. The immune complexes or conjugates were efficiently bound on the mimetic Blue A6XL column and eluted under mild conditions (5-20 mM phosphate buffer). A similar strategy of purifying peroxidase/anti-peroxidase antibody complexes was also successfully demonstrated using the mimetic Red 3 column. Mimetic affinity chromatography thus appears to be a simple method to purify the desired monospecific or bispecific antibodies from the respective hybridomas and quadromas.

References

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Citations

Feb 28, 2001·Journal of Immunological Methods·D R Husereau, M R Suresh
Jun 29, 2002·Journal of Biochemical and Biophysical Methods·Seema Gupta, Mavanur Suresh
Jul 16, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yang LiuGuoquan Liu
Feb 9, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pravin K BhatnagarMavanur R Suresh

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