Mimic Peroxidase- and Bi2 S3 Nanorod-Based Photoelectrochemical Biosensor for Signal-On Detection of Polynucleotide Kinase

Analytical Chemistry
Lin CuiChun-Yang Zhang

Abstract

We demonstrate for the first time the development of a mimic peroxidase- and bismuth sulfide (Bi2S3) nanorod-based photoelectrochemical (PEC) biosensor for signal-on detection of polynucleotide kinase (PNK) on the basis of manganese-based mimic enzyme (MnME) catalytic precipitation. We use the hybrid film which consists of Bi2S3 nanorods and Au nanoparticles (AuNPs) as the immobilization matrix of capture probe. The capture probe on the modified electrode can specifically hybridize with the MnME@AuNPs-labeled signal probe to form the double-stranded DNA (dsDNA), generating a PEC biosensor. In the absence of PNK, MnME may stimulate the mimic enzyme catalytic precipitation onto the electrode surface, blocking the interfacial electron transfer and eventually leading to a low PEC signal. While in the presence of PNK, the dsDNA is phosphorylated and subsequently cleaved by lambda exonuclease to release the MnME@AuNPs conjugates from the electrode, leading to the decrease of catalytic precipitation on the surface of electrode and consequently the production of a high PEC signal. Notably, the MnME can be easily synthesized and possesses higher catalytic activity than the manganese-based mimic enzyme. This signal-on PEC biosensor exhib...Continue Reading

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Citations

Nov 14, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lan GeWang Kun
Jun 25, 2020·Sensors·Chunmei LiXuemei Wang
Jan 29, 2021·The Analyst·Renzhong YuZhihui Dai
Nov 5, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Krishna ThapaRisheng Wang

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