Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources

Scientific Reports
Jun NiPing Xu

Abstract

Plant secondary metabolites have been attracting people's attention for centuries, due to their potentials; however, their production is still difficult and costly. The rich diversity of microbes and microbial genome sequence data provide unprecedented gene resources that enable to develop efficient artificial pathways in microorganisms. Here, by mimicking a natural pathway of plants using microbial genes, a new metabolic route was developed in E. coli for the synthesis of vanillin, the most widely used flavoring agent. A series of factors were systematically investigated for raising production, including efficiency and suitability of genes, gene dosage, and culture media. The metabolically engineered strain produced 97.2 mg/L vanillin from l-tyrosine, 19.3 mg/L from glucose, 13.3 mg/L from xylose and 24.7 mg/L from glycerol. These results show that the metabolic route enables production of natural vanillin from low-cost substrates, suggesting that it is a good strategy to mimick natural pathways for artificial pathway design.

References

Oct 12, 2000·Current Opinion in Biotechnology·N J WaltonG Williamson
Sep 11, 2001·Applied Microbiology and Biotechnology·H PriefertA Steinbüchel
May 10, 2002·Nucleic Acids Research·David M Hoover, Jacek Lubkowski
Aug 30, 2002·Applied and Environmental Microbiology·Eiji MasaiMasao Fukuda
Jun 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Alain GoossensKirsi-Marja Oksman-Caldentey
Jun 9, 2004·Chembiochem : a European Journal of Chemical Biology·Kevin T WattsClaudia Schmidt-Dannert
Oct 6, 2005·Bioresource Technology·V J MehtaS P Singh
Mar 21, 2006·Journal of Bacteriology·Martin BernerAndreas Bechthold
Jun 2, 2006·Molecular Systems Biology·Ernesto AndrianantoandroRon Weiss
Sep 2, 2006·Biotechnology and Bioengineering·Tyler W JohannesHuimin Zhao
Sep 12, 2006·Protein Expression and Purification·Seonghun Kim, Sun Bok Lee
Dec 20, 2007·Molecular Systems Biology·Luis Serrano
Jan 23, 2009·Journal of the American Chemical Society·Joseph B Binder, Ronald T Raines
Mar 17, 2009·Applied and Environmental Microbiology·Esben H HansenJørgen Hansen
Sep 1, 2002·Molecular Plant Pathology·Richard A DixonLiangjiang Wang
Nov 10, 2010·Microbial Cell Factories·Ana Rita BrochadoKiran Raosaheb Patil
Feb 16, 2011·Metabolic Engineering·Christine Nicole S SantosGregory Stephanopoulos
Mar 23, 2011·Journal of Industrial Microbiology & Biotechnology·Oksik ChoiYoung-Soo Hong
Apr 23, 2011·Journal of Industrial Microbiology & Biotechnology·Ana Joyce MuñozGuillermo Gosset
May 26, 2011·Nature Communications·Akira NakagawaHidehiko Kumagai
Apr 6, 2012·Microbial Cell Factories·Yuheng Lin, Yajun Yan
Nov 28, 2012·Applied Microbiology and Biotechnology·Haoran Zhang, Gregory Stephanopoulos
Jan 12, 2013·Applied Biochemistry and Biotechnology·Baljinder Kaur, Debkumar Chakraborty
Jun 20, 2014·Nature Communications·Nethaji J GallageBirger Lindberg Møller
Jul 31, 2014·Journal of the American Chemical Society·Aditya M KunjapurKristala L J Prather

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Citations

Dec 8, 2017·Biotechnology for Biofuels·Jason T KuEthan I Lan
Aug 11, 2018·Journal of the Science of Food and Agriculture·Goutam Banerjee, Pritam Chattopadhyay
Sep 23, 2018·Applied and Environmental Microbiology·Ashley M FrankAlison Buchan
May 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laure-Anne PeyratNikolas Fokialakis
Mar 31, 2017·Planta·Anish Kundu
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Paulette Gómez-LópezRafael Luque
Apr 22, 2020·Nucleic Acids Research·Shaozhen DingQian-Nan Hu
Jan 4, 2021·Biotechnology Advances·Lichao SunHal S Alper
Mar 21, 2020·Biotechnology Advances·Yasin Torres-TijiStephen P Mayfield
Jul 20, 2021·Green Chemistry : an International Journal and Green Chemistry Resource : GC·Joanna C Sadler, Stephen Wallace
Mar 14, 2019·ACS Synthetic Biology·Min Yan TehMeng How Tan
Oct 31, 2018·Journal of the American Chemical Society·Jun NiPing Xu
Sep 17, 2021·Journal of Agricultural and Food Chemistry·Bixia FuJifeng Yuan

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Methods Mentioned

BETA
glycosylation
deamination
FCS
reverse transcription PCR
genetic modification
PCR

Software Mentioned

SPSS
Office Excel
Beacon Designer

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