Mimicking physiological O2 tension in the female reproductive tract improves assisted reproduction outcomes in pig

Molecular Human Reproduction
S García-MartínezO López Albors

Abstract

Is O2 tension in the pig oviduct and uterus affected by the estrous cycle stage and the animal's age, and can the outcome of in vitro embryo development be improved by mimicking these physiological values? O2 tension within the pig reproductive organs is affected by the animal's age, and values close to those measured in vivo have a positive impact on embryo development and quality when used during IVF and embryo culture (EC). To obtain a healthy embryo in vitro, it is necessary to adopt a culture microenvironment that approximates physiological conditions. Despite advances in surgical procedures and sensitive probes that allow accurate assessment of in vivo O2 tension, few such studies have been conducted recently in mammals. In addition, no reference values of physiological O2 tension in the reproductive tract exist for large animal models such as pig, and the effect of O2 tension on ART outcomes is unknown. This study was conducted in pigs. We measured oviductal and uterine O2 tension (n = 29 and 13, respectively) and then examined how the use of the physiological values in pig IVF and EC affected pig ART output (n = 1447 oocytes). The oviductal and uterine O2 tension at the different stages of the estrous cycle was monitore...Continue Reading

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Citations

Apr 29, 2020·Zygote : the Biology of Gametes and Early Embryos·M JamilR Cadi
Aug 11, 2020·Zygote : the Biology of Gametes and Early Embryos·Ruizhe LiShengmei Chen
Sep 4, 2019·Molecular Reproduction and Development·Caroline G LucasTiago Collares
Jun 28, 2019·Reproduction, Fertility, and Development·M S LorenzoD M Lombardo
Jul 30, 2020·Animals : an Open Access Journal From MDPI·Ximo Garcia-DominguezFrancisco Marco-Jiménez
Aug 13, 2020·Animals : an Open Access Journal From MDPI·Jordana S LopesPilar Coy
May 10, 2020·Animals : an Open Access Journal From MDPI·Ximo Garcia-DominguezFrancisco Marco-Jiménez
Nov 20, 2020·International Journal of Molecular Sciences·Francisco Marco-JiménezJosé S Vicente
Feb 6, 2021·Scientific Reports·Octavio López-AlborsFrancisco Alberto García-Vázquez
Feb 25, 2021·Zygote : the Biology of Gametes and Early Embryos·Nasser GhanemAshraf Hesham Barkawi

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