Mimivirus TyrRS: preliminary structural and functional characterization of the first amino-acyl tRNA synthetase found in a virus

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Chantal AbergelJ M Claverie

Abstract

The amoeba-infecting Mimivirus is the largest known double-stranded DNA virus, with a 400 nm particle size, comparable to that of mycoplasma. The complete sequence of its 1.2 Mbp genome has recently been determined [Raoult et al. (2004), Science, 306, 1344-1350] and revealed numerous genes that were not expected to be found in a virus, such as genes encoding translation components, including 4-amino-acyl tRNA synthetases and homologues to various translation initiation, elongation and termination factors. A comprehensive structural and functional study of these Mimivirus gene products was initiated, as they may hold important clues about the origin of DNA viruses. Here, the first preliminary crystallographic and functional results obtained on one of these targets, Mimivirus TyrRS, are reported. Preliminary phasing was obtained using an original combination of homology modelling and normal mode analysis. Experimental evidence that Mimivirus tyrosyl tRNA synthetase recombinant gene product does indeed activate tyrosine is also presented.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Nov 15, 2000·Genome Research·J L HartleyM A Brasch
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·J Navaza
Nov 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiang-Lei YangPaul Schimmel
Mar 29, 2003·Science·Bernard La ScolaDidier Raoult
May 20, 2003·Nature Structural Biology·Takatsugu KobayashiShigeyuki Yokoyama
Mar 25, 2004·Acta Crystallographica. Section D, Biological Crystallography·Karsten Suhre, Yves Henri Sanejouand
Jun 25, 2004·Nucleic Acids Research·Olivier PoirotCedric Notredame
Jun 25, 2004·Nucleic Acids Research·Jean-Baptiste ClaudeChantal Abergel
Oct 16, 2004·Science·Didier RaoultJean-Michel Claverie

❮ Previous
Next ❯

Citations

Sep 8, 2006·Journal of Bioenergetics and Biomembranes·Sandra JeudyChantal Abergel
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sandra JeudyChantal Abergel
Apr 3, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Luc BonnefondClaude Sauter
Aug 6, 2009·Annual Review of Genetics·Jean-Michel Claverie, Chantal Abergel
Oct 18, 2008·The International Journal of Biochemistry & Cell Biology·Natalia WegnerPatrick J Venables
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·You-Na KangSteven E Ealick
Jan 7, 2015·Archives of Biochemistry and Biophysics·Swapnil Mahajan, Yves-Henri Sanejouand
Feb 17, 2017·PLoS Genetics·Jônatas Santos AbrahãoBernard La Scola
Dec 22, 2006·Molecular Biology and Evolution·Björn BrindefalkSiv G E Andersson
Apr 26, 2020·Archives of Virology·Rodrigo Araújo Lima RodriguesJônatas Santos Abrahão
Apr 13, 2018·Virology·Christopher R Schvarcz, Grieg F Steward

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.