Mimotopes of polyreactive anti-DNA antibodies identified using phage-display peptide libraries

European Journal of Immunology
P SibilleA Avrameas

Abstract

Three monoclonal IgG2a anti-DNA polyreactive autoantibodies, derived from lupus-prone mice (NZB x NZW)F1, were studied by surface plasmon resonance (BIAcore) analysis using three different synthetic double-stranded (ds) oligonucleotides of 25, 30, and 25 base pairs (bp). These monoclonal antibodies (mAb) exhibited dissociation rate constants (k(off)), ranging from 0.0001 (mAb F14.6 and F4.1) to 0.01/s (mAb J20.8) and k(on) ranging from 2 x 10(5) to 2 x 10(6) /M/s. The screening of a constrained random peptide library displayed on M13 bacteriophages on these mAb allowed the determination of the specific consensus motifs (mimotopes) for mAb F14.6 and J20.8, but not for mAb F4.1. No cross-reaction was observed between F14.6- and J20.8-specific peptides (and vice versa). Binding of all phages selected on F14.6 was inhibited with 700 ng/ml soluble DNA. The binding of one group of peptides selected on J20.8 was inhibited by 400 ng/ml soluble DNA, of a second group by 2500 ng/ml, while binding of a third group could not be inhibited. The determined consensus sequences do not match with known sequences. Peptides specific for F14.6 share negative charges and aromatic rings that may mimic a DNA backbone, while peptides selected with J20....Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K R OldenburgM A Gallop
Jul 27, 1990·Science·J K Scott, G P Smith
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B D StollarR W Pastor
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y OhosoneJ A Hardin
Feb 1, 1993·European Journal of Immunology·E RazD Eilat
Jan 1, 1993·Methods in Enzymology·G P Smith, J K Scott
Aug 1, 1994·Journal of Autoimmunity·B HentatiT Ternynck
Jan 1, 1994·The Journal of Clinical Investigation·M ReichlinB H Hahn
Jun 25, 1993·Nucleic Acids Research·Z Yang, J D Engel

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Citations

Oct 23, 2001·Arthritis and Rheumatism·G RiemekastenF Hiepe
Jan 26, 2006·Anatomy and Embryology·S HolmsethN C Danbolt
Mar 24, 2005·Journal of Molecular Biology·Jonathan P SchuermannJohn J Tanner
Mar 1, 2000·Journal of Neuroimmunology·J X Qiu, D M Marcus
Jul 27, 1999·Combinatorial Chemistry & High Throughput Screening·G CesareniG Cestra
Jan 5, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Silvia HolmsethNiels Christian Danbolt
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·A AvrameasS Avrameas
Oct 21, 2015·Clinical and Experimental Immunology·Y XiaC Putterman
Jan 11, 2011·Molecular Immunology·Joel Cohen-Solal, Betty Diamond
May 3, 2003·Annals of the New York Academy of Sciences·Kenneth M KaufmanJudith A James
Jun 26, 2008·Arthritis and Rheumatism·Casandra C van BavelJo H Berden
Oct 27, 2005·Journal of Autoimmunity·Riccardo NavoneAntonio Puccetti
Apr 23, 2008·Current Protocols in Neuroscience·A Bradbury
Apr 8, 2010·Current Protocols in Neuroscience·Andrew R M Bradbury
Jul 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Venkatasamy ManivelKanury V S Rao
Mar 30, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeganathan VenkateshBetty Diamond
Jan 20, 1999·Bioconjugate Chemistry·A AvrameasG Buttin
May 8, 2014·The Journal of Physical Chemistry. B·Yi AnSteven E Wheeler

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