MIN6 beta-cell-beta-cell interactions influence insulin secretory responses to nutrients and non-nutrients

Biochemical and Biophysical Research Communications
Melanie J LutherPeter M Jones

Abstract

Insulin-secreting MIN6 cells show greatly enhanced secretory responsiveness to nutrients when grown as islet-like structures (pseudoislets). Since beta-cells use different mechanisms to respond to nutrient and non-nutrient stimuli, we have now investigated the role of homotypic beta-cell interactions in secretory responses to pharmacological or receptor-operated non-nutrient stimuli in MIN6 pseudoislets. In addition to an enhanced secretory responsiveness to glucose, insulin secretion from MIN6 pseudoislets was also enhanced by non-nutrients, including carbachol, tolbutamide, PMA, and forskolin. The improved secretory responsiveness was dependent on the cells being configured as pseudoislets and was lost on dispersal of the pseudoislets into single cells and regained on the re-formation of pseudoislet structures. These observations emphasise the importance of islet anatomy on secretory responsiveness, and demonstrate that homotypic beta-cell interactions play an important role in generating physiologically appropriate insulin secretory responses to both nutrient and non-nutrient stimuli.

References

Sep 1, 1990·The Journal of Clinical Investigation·P MedaL Orci
Sep 1, 1985·Endocrinology·D G PipeleersW Gepts
May 1, 1987·Diabetologia·D Pipeleers
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D PipeleersM Van De Winkel
Dec 1, 1995·The Journal of Cell Biology·C VozziP Meda
Jan 1, 1997·Advances in Experimental Medicine and Biology·P SmithF M Ashcroft
Jan 1, 1997·Advances in Experimental Medicine and Biology·F C SchuitH Heimberg
Jan 1, 1997·Advances in Experimental Medicine and Biology·D Bosco, P Meda
Feb 26, 1999·The Journal of Biological Chemistry·C A AspinwallR T Kennedy
Jun 14, 2002·Molecular and Cellular Endocrinology·A C Hauge-EvansP M Jones
Oct 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Ingmar LundquistBo Hellman
Mar 17, 2004·Experimental Cell Research·Alessandra CalabresePaolo Meda
Oct 14, 2004·American Journal of Physiology. Endocrinology and Metabolism·Melanie J LutherPeter M Jones

❮ Previous
Next ❯

Citations

May 11, 2012·Biomedical Microdevices·Daniel Gallego-PerezKeith J Gooch
Aug 11, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ortwin NaujokSigurd Lenzen
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chien-Chi Lin, Kristi S Anseth
Aug 30, 2008·Tissue Engineering. Part a·Laney M WeberKristi S Anseth
Feb 11, 2012·Tissue Engineering. Part C, Methods·Abigail B BernardKristi S Anseth
Jul 18, 2008·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Quan ZhangPatrik Rorsman
Nov 6, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Gareth J RogersPaul E Squires
Sep 10, 2013·PloS One·Michael G SpeliosEitan M Akirav
Aug 19, 2014·Cell and Tissue Research·Xiaofang LiuXuetao Pei
Jun 21, 2012·Canadian Journal of Physiology and Pharmacology·Junia Carolina Santos-SilvaCarla Beatriz Collares-Buzato
Sep 14, 2007·Archives of Physiology and Biochemistry·Peter M JonesShanta J Persaud
Jan 9, 2014·Journal of Tissue Engineering and Regenerative Medicine·O NaujokS Lenzen
Mar 5, 2014·Organogenesis·Nobuhiko Kojima
Jul 20, 2010·Biochemical and Biophysical Research Communications·Catriona KellyNeville H McClenaghan
Sep 15, 2016·International Journal of Molecular Sciences·Milou Groot NibbelinkAart Van Apeldoorn
Sep 29, 2011·Cell Transplantation·E MaillardS Sigrist
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Melanie J CarvellPeter M Jones
Jan 26, 2008·Endocrinology·Fabienne JaquesPhilippe A Halban
May 9, 2019·Stem Cells Translational Medicine·Ahmed A ArzouniPeter M Jones
Aug 14, 2019·Journal of Biomedical Materials Research. Part a·Mehdi RazaviAvnesh S Thakor
Aug 9, 2017·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Stephanie R VillaBrian T Layden
Oct 13, 2010·Biotechnology and Bioengineering·Corinne A HoesliJames M Piret
Apr 26, 2015·The Journal of Biological Chemistry·Michael G SpeliosEitan M Akirav
Feb 14, 2013·The International Journal of Artificial Organs·Matthew L SkilesJames O Blanchette
Mar 18, 2015·Journal of Cellular and Molecular Medicine·Janneke HilderinkAart van Apeldoorn

❮ Previous
Next ❯

Related Concepts

Related Feeds