Minced urothelium to create epithelialized subcutaneous conduits

The Journal of Urology
Magdalena FossumElof Eriksson

Abstract

We used in vivo cell expansion to create 3-dimensional subcutaneous conduits lined with an inner layer of autologous urothelial mucosa. Laparotomy and excision of a fifth of the bladder were done in 5 female Yorkshire pigs (Parsons Farm, Westhampton, Massachusetts) under general anesthesia. After mechanical removal of the detrusor muscle the bladder mucosa was minced to obtain 0.2 x 0.8 x 0.8 mm particles, which were attached to the outer surface of latex tubes using a thin layer of fibrin glue. Seven to 10 tubes were placed in the abdominal wall subcutaneous tissue in each original donor pig with tubes lacking particles serving as controls. Biopsy was done 1 to 4 weeks after transplantation for histological evaluation. One week after transplantation particles were still present in the granulation tissue. At 2 weeks the epithelium was differentiated with transitional uroepithelium facing the lumen, ie toward the tube. No epithelium was detected around control tubes. After autologous transplantation of bladder mucosal particles organized in 3-dimensional fashion in pig subcutaneous tissue the transplanted cells proliferated, migrated and reorganized to form a continuous epithelial lining facing the lumen. This novel approach to ...Continue Reading

References

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Mar 19, 2009·Tissue Engineering. Part a·Magdalena FossumElof Eriksson
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Citations

Jan 19, 2013·Tissue Engineering. Part C, Methods·Fatemeh AjalloueianJöns Hilborn
Jun 19, 2012·The Journal of Urology·Paul GeutjesWout Feitz
Nov 30, 2013·BioMed Research International·Gisela Reinfeldt EngbergMagdalena Fossum
May 18, 2016·The Journal of Urology·Marije SloffWout F J Feitz
Oct 26, 2016·BioMed Research International·Gisela Reinfeldt EngbergMagdalena Fossum
Feb 24, 2017·Journal of Tissue Engineering·Rhea SaksenaAchala de Mel
Dec 28, 2018·Expert Review of Medical Devices·Jan AdamowiczTomasz Drewa
Aug 20, 2021·Scientific Reports·Arkadiusz JundziłłTomasz Drewa

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