MinD and MinE interact with anionic phospholipids and regulate division plane formation in Escherichia coli

The Journal of Biological Chemistry
Lars D Renner, Douglas B Weibel

Abstract

The Min proteins (MinC, MinD, and MinE) form a pole-to-pole oscillator that controls the spatial assembly of the division machinery in Escherichia coli cells. Previous studies identified that interactions of MinD with phospholipids positioned the Min machinery at the membrane. We extend these studies by measuring the affinity, kinetics, and ATPase activity of E. coli MinD, MinE, and MinDE binding to supported lipid bilayers containing varying compositions of anionic phospholipids. Using quartz crystal microbalance measurements, we found that the binding affinity (K(d)) for the interaction of recombinant E. coli MinD and MinE with lipid bilayers increased with increasing concentration of the anionic phospholipids phosphatidylglycerol and cardiolipin. The K(d) for MinD (1.8 μM) in the presence of ATP was smaller than for MinE (12.1 μM) binding to membranes consisting of 95:5 phosphatidylcholine/cardiolipin. The simultaneous binding of MinD and MinE to membranes revealed that increasing the concentration of anionic phospholipid stimulates the initial rate of adsorption (k(on)). The ATPase activity of MinD decreased in the presence of anionic phospholipids. These results indicate that anionic lipids, which are concentrated at the p...Continue Reading

References

Jun 13, 1986·Biochimica Et Biophysica Acta·L D MayerP R Cullis
Feb 11, 1972·Biochimica Et Biophysica Acta·R P Rand, S Sengupta
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·David M Raskin, Piet A J de Boer
Sep 16, 2000·Physical Review Letters·C A KellerB Kasemo
Sep 22, 2001·Journal of Bacteriology·C M KoppelmanN Nanninga
Oct 24, 2001·Journal of Bacteriology·T H SzetoGlenn F King
Apr 16, 2002·The EMBO Journal·Brian D CorbinWilliam Margolin
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zonglin HuJoe Lutkenhaus
Nov 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tim H SzetoGlenn F King
Dec 24, 2002·Biophysical Journal·Joseph M JohnsonSteven G Boxer
Jan 21, 2003·Journal of Bacteriology·Laura L LacknerPiet A J de Boer
Apr 5, 2003·The Journal of Biological Chemistry·Eugenia MileykovskayaWilliam Dowhan
Jul 29, 2003·The Journal of Biological Chemistry·Tim H SzetoGlenn F King
Oct 7, 2003·Annual Review of Microbiology·John E Cronan
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kerwyn Casey HuangNed S Wingreen
Oct 6, 2005·Physical Biology·Kerwyn Casey Huang, Ned S Wingreen
Jul 19, 2006·PLoS Computational Biology·David Fange, Johan Elf
Feb 17, 2007·Molecular Microbiology·Karsten KruseWilliam Margolin
May 8, 2007·Biophysical Journal·Eric N Cytrynbaum, Brandon D L Marshall
May 17, 2007·Molecular Microbiology·Tatyana RomantsovJanet M Wood
Apr 24, 2008·Molecular Microbiology·Imrich BarákNada Pavlendová
Aug 12, 2008·Biophysical Journal·Nada KhalifatMiglena I Angelova
Sep 2, 2008·Biochimica Et Biophysica Acta·Shirley MazorItzhak Fishov
Nov 4, 2009·Current Opinion in Microbiology·Marc Bramkamp, Suey van Baarle
Dec 23, 2009·Molecular Microbiology·Cheng-Wei HsiehYu-Ling Shih
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vassili Ivanov, Kiyoshi Mizuuchi
May 7, 2010·Molecular Microbiology·Kerwyn Casey Huang, Kumaran S Ramamurthi
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lars D Renner, Douglas B Weibel
Apr 26, 2011·Nature Structural & Molecular Biology·Martin LoosePetra Schwille
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jakob SchweizerPetra Schwille

Citations

Jun 7, 2013·Journal of the American Chemical Society·Ye-Jin EunDouglas B Weibel
Dec 18, 2013·PLoS Computational Biology·Mike BonnyKarsten Kruse
Dec 19, 2013·Journal of Cell Science·Géraldine Laloux, Christine Jacobs-Wagner
Feb 26, 2016·Current Opinion in Cell Biology·Simon Kretschmer, Petra Schwille
Aug 15, 2015·FEBS Letters·Samuel FurseJ Antoinette Killian
Feb 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Anthony G VecchiarelliKiyoshi Mizuuchi
Apr 12, 2014·Molecular Microbiology·Thiago M A SantosDouglas B Weibel
Jun 17, 2014·Molecular Microbiology·Anthony G VecchiarelliKiyoshi Mizuuchi
Jun 30, 2015·Environmental Microbiology·Meriyem Aktas, Franz Narberhaus
Mar 31, 2016·Applied Microbiology and Biotechnology·Ti-Yu Lin, Douglas B Weibel
Feb 18, 2014·Current Opinion in Microbiology·Zachary D Dalebroux, Samuel I Miller
Jan 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Zachary D DalebrouxSamuel I Miller
Aug 11, 2016·Biochemistry·Samuel Furse, David J Scott
Sep 23, 2016·Pharmaceuticals·Nermina Malanovic, Karl Lohner
Nov 26, 2016·ELife·Yaron Caspi, Cees Dekker
Apr 11, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lukas Wettmann, Karsten Kruse
Jul 25, 2018·Biotechnology Journal·Masayoshi TanakaTadashi Matsunaga
Aug 31, 2018·PloS One·Lukas WettmannKarsten Kruse
Jul 9, 2014·Journal of Bacteriology·Piercen M OliverDouglas B Weibel
Oct 6, 2018·Bioscience, Biotechnology, and Biochemistry·Lei KeJian-Chun Guo
Feb 21, 2019·Journal of Bacteriology·Julia F NepperDouglas B Weibel
Jun 10, 2016·Molecular Systems Biology·Fabai WuCees Dekker
Dec 1, 2017·Physical Biology·Kiyoshi Mizuuchi, Anthony G Vecchiarelli
Nov 29, 2016·Molecular Microbiology·Joshua S MacCreadyDaniel C Ducat
Feb 1, 2019·Journal of Cell Science·Kim J A VendelLiedewij Laan
Nov 9, 2019·Physical Review Letters·Andriy Goychuk, Erwin Frey
May 18, 2016·The Journal of Biological Chemistry·Guillaume SautreyMarie-Paule Mingeot-Leclercq
Jan 11, 2020·Frontiers in Cell and Developmental Biology·Mikhail A ZhukovskyCarmen Valente
Jul 19, 2019·Cellular and Molecular Life Sciences : CMLS·Beatrice RammPetra Schwille
Jan 20, 2017·Journal of Chemical Biology·Samuel Furse
Jan 12, 2021·Critical Reviews in Microbiology·Reema ChaudharyHari Misra

Related Concepts

MinD protein, E coli
MinE protein, E coli
DNA-dependent ATPase
Adsorption
Antibiotics
Diphosphatidylglycerols
Cell Division Phases
Dose-Response Relationship, Drug
Alkalescens-Dispar Group
Lipid Bilayers

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.