PMID: 9435125Jan 22, 1998Paper

Mini-exon gene sequences define six groups within the genus Crithidia

The Journal of Eukaryotic Microbiology
O FernandesD A Campbell

Abstract

To develop molecular markers for lower trypanosmatids, we have examined the mini-exon gene repeats of 17 isolates that were classified as Crithidia by traditional methods. Representative repeats were amplified by polymerase chain reaction and the amplification products were cloned and used as hybridization probes against genomic DNA. Six hybridization groups of Crithidia were defined on the basis of the DNA blotting experiments. The three endosymbiont-bearing species (C. deanei, C. desouzai and C. oncopelti) and C. acanthocephali each belonged to single-member hybridization groups, while the C. fasciculata group contained additional named and undesignated species. The Crithidia lucilae thermophila probe hybridized to multiple undesignated isolates. The DNA sequence of the cloned products revealed that the specificity of the hybridization probes was due to substantial differences in the intron and the nontranscribed spacer regions. These data indicate substantial heterogeneity within the mini-exon gene locus of the taxon Crithidia.

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Citations

Jan 16, 1999·Molecular and Cellular Biology·N R SturmD A Campbell
Jun 29, 2004·The Journal of Eukaryotic Microbiology·Sergei A PodlipaevJulius Lukes
Feb 3, 2007·International Journal of Systematic and Evolutionary Microbiology·Milena SvobodováJan Votýpka
Jun 20, 2001·The Journal of Eukaryotic Microbiology·N R SturmD A Campbell
Aug 1, 2012·The Journal of Eukaryotic Microbiology·Milan JirkůDmitri A Maslov
May 4, 1999·Memórias do Instituto Oswaldo Cruz·E C GrisardA J Romanha
Jul 25, 2000·Memórias do Instituto Oswaldo Cruz·S A Podlipaev
Feb 13, 2001·Journal of Invertebrate Pathology·M A EbbertJ L Marlowe
Oct 19, 2007·European Journal of Protistology·André Luis Souza dos SantosLucimar Ferreira Kneipp

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