Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii

Journal of Microbiological Methods
Ayush KumarHerbert P Schweizer

Abstract

Acinetobacter baumannii is an emerging pathogen that causes serious infections with high mortality rates in immunocompromised individuals. Genetic manipulations in this medically-relevant pathogen are limited by the paucity of molecular tools. In this study, we show the application of the mini-Tn7-based single copy insertion system in A. baumannii. Mini-Tn7 elements are known to integrate at a naturally evolved, therefore presumably neutral location (intergenic region) downstream of the glmS gene (glucosamine-fructose-6-phosphate aminotransferase) in Gram-negative bacteria. We identified the site of insertion of mini-Tn7 in A. baumannii and demonstrated application of this useful cloning tool by inserting the gfp gene into the chromosome. Our work shows that mini-Tn7 elements are useful tools for genetic studies in this important pathogen.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C S Waddell, N L Craig
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Apr 4, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G BouJ Martínez-Beltrán
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·J E Peters, N L Craig
May 24, 2005·Nature Methods·Kyoung-Hee ChoiHerbert P Schweizer
Aug 30, 2005·Antimicrobial Agents and Chemotherapy·Richard J GillisBarbara H Iglewski
Apr 5, 2007·Nature Protocols·Kyoung-Hee Choi, Herbert P Schweizer
Feb 9, 2008·Emerging Infectious Diseases·James W T ElstonCraig S Boutlis
Mar 26, 2008·Critical Care Clinics·Clinton K Murray, Duane R Hospenthal

❮ Previous
Next ❯

Citations

Mar 26, 2013·PloS One·Michael D CarruthersRobert S Munson
May 8, 2015·Journal of Medical Microbiology·Indranil Biswas
Sep 30, 2015·BMC Microbiology·Lorena Romero-JiménezDaniel Pérez-Mendoza
Feb 5, 2015·BMC Microbiology·Steven T BruckbauerHerbert P Schweizer
Feb 14, 2015·PLoS Pathogens·Edward Geisinger, Ralph R Isberg
Jul 20, 2016·Antimicrobial Agents and Chemotherapy·James M RegeimbalEric R Hall
Apr 8, 2015·Journal of Bacteriology·Larry A GallagherColin Manoil
Feb 26, 2016·Scientific Reports·Suleyman YildirimBenjamin C Kirkup
Nov 5, 2014·Current Protocols in Microbiology·Anna C JacobsDaniel V Zurawski
Jan 4, 2020·The Journal of Microbiology·Eun Kyung LeeMan Hwan Oh
Mar 3, 2020·Frontiers in Microbiology·Caitlin L WilliamsD Scott Merrell
Jan 9, 2021·Frontiers in Genetics·Ellen M E SykesAyush Kumar
Jul 8, 2021·Applied and Environmental Microbiology·Félix BornierXavier Charpentier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.