Miniaturization of frit inlet asymmetrical flow field-flow fractionation

Analytical Chemistry
Dukjin Kang, Myeong Hee Moon

Abstract

A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of sample materials. The end effect contribution to plate height was evaluated by varying the inner diameter of the connection tubing between the injector and the channel inlet at various injection flow rates, and the results showed that the use of silica capillary tubing of the shortest possible distance is essential in reducing the initial band broadening prior to the sample injection to the microscale channel. The capability of the microFI-AFlFFF system was demonstrated with the separation of protein standards, polystyrenesulfonates, and ssDNA strains and for the characterization of replication protein A-ssDNA binding complex regulated by redox status.

Citations

Feb 17, 2009·Analytica Chimica Acta·Barbara RodaAldo Roda
May 22, 2013·Journal of Chromatography. a·Samantha SchachermeyerWenwan Zhong
Jul 8, 2008·Journal of Proteomics·Pierluigi Reschiglian, Myeong Hee Moon
Aug 11, 2015·Frontiers in Chemistry·David MüllerAndrew J de Mello
Jul 30, 2014·Journal of Chromatography. a·Jonathan AshbyWenwan Zhong
Dec 22, 2005·Journal of Chromatography. a·Himanshu J Sant, Bruce K Gale
Jun 15, 2007·Journal of Separation Science·Sunok OhMyeong Hee Moon
Dec 29, 2016·Journal of Chromatography. a·Ugo TillAnne-Françoise Mingotaud
May 9, 2013·Analytical Chemistry·Tyler N ShendrukMichel Godin

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