Abstract
Fabrication and characterization of miniaturized amperometric hypoxanthine biosensors are described and demonstrated for monitoring hypoxanthine in myocardial cell culture media. The sensors are based on xanthine oxidase (XO) immobilized on carbon fiber microelectrodes (CFMEs) using a composite film of Nafion and electropolymerized phenol (PPh). Nafion was used for XO immobilization because of its film hydrophobicity, enzyme-favored environment, and electrostatic interaction with XO, which was dispersed in Nafion film by immersing the Nafion-coated CFMEs in XO solution for 5 h. PPh film was formed as an overlay on Nafion and XO-modified CFMEs via electropolymerization. Hypoxanthine was measured with the sensor by the oxidation of enzymatic reaction products, hydrogen peroxide (H(2)O(2)), and uric acid (UA) at +0.60 V (vs Ag/AgCl). The use of Nafion and PPh as a matrix for XO immobilization yields enhanced specificity, sensitivity, and linearity toward hypoxanthine. A dynamic linear range of 5.0 microM to 1.8 mM was achieved with a calculated detection limit of 1.5 microM (S/N = 3) and a sensitivity of 3.144 nA/mM. In addition, the measurement was virtually interference-free from easily oxidizable species such as UA, ascorbic ac...Continue Reading
References
Jan 1, 1987·The Analyst·G J MoodyJ D Thomas
Jan 1, 1994·Biosensors & Bioelectronics·J HlavayG G Guilbault
Jan 1, 1994·Biosensors & Bioelectronics·M RehákM Otto
Jul 1, 1996·The Annals of Thoracic Surgery·D E Van RaemdonckT E Lerut
Apr 12, 1996·Journal of Chromatography. B, Biomedical Applications·K F ChildsS F Bolling
Jan 1, 1996·Life Sciences·G Biessels, W H Gispen
Dec 1, 1996·Journal of Neuroscience Methods·M Miele, M Fillenz
Jun 1, 1997·Journal of Pharmaceutical and Biomedical Analysis·T SekiA Shinoda
Apr 16, 1998·Cardiovascular Research·S A MantheiD G Van Wylen
May 23, 1998·Advances in Experimental Medicine and Biology·W Makarewicz
Mar 18, 1999·Analytical Chemistry·T NakaminamiH Yoneyama
May 3, 2000·Analytical Biochemistry·X Jing-Juan, C Hong-Yuan
Oct 3, 2000·Analytical Chemistry·B WangS Dong
Oct 6, 2000·Analytical Biochemistry·M StredanskyJ Svorc
Nov 18, 2000·Analytical Chemistry·J XueL Jin
Jun 3, 1960·Science·I HARARY, B FARLEY
Jul 1, 1996·Talanta·S Pan, M A Arnold
Dec 1, 1996·Talanta·M NumataY Yano
Oct 31, 2008·Talanta·M A CarsolM Mascini
Oct 31, 2008·Talanta·M A Carsol, M Mascini
Citations
Mar 19, 2014·Enzyme and Microbial Technology·Chandra Shekhar Pundir, Rooma Devi
Dec 3, 2014·Analytica Chimica Acta·Tânia S C R RebeloM H Fernandes
Oct 6, 2005·Biosensors & Bioelectronics·Yang TianTakeo Ohsaka
Mar 22, 2015·Food Chemistry·Muamer DervisevicMehmet Şenel
Nov 13, 2012·Talanta·Abdulazeez T Lawal, Samuel B Adeloju
Nov 2, 2004·Analytical and Bioanalytical Chemistry·Fen-Fen ZhangKatsunobu Yamamoto
Feb 24, 2009·Biochemistry. Biokhimii︠a︡·M R RashidiA Jouyban
Dec 25, 2012·Analytical and Bioanalytical Chemistry·A Carolina TorresChristopher M A Brett
Jul 28, 2019·Bioelectrochemistry·Buket Yalcin SahyarSemih Otles
May 31, 2017·Enzyme and Microbial Technology·Ali HalalipourJosé I Reyes-De-Corcuera
Nov 22, 2011·Talanta·Salih Zeki BaşAhmet Gülce
May 1, 2009·Analytical Chemistry·Stanislav HasonFrantisek Jelen
Mar 3, 2011·The Journal of Physical Chemistry. B·Palraj KalimuthuPaul V Bernhardt
Sep 1, 2012·The Journal of Physical Chemistry. B·Palraj KalimuthuPaul V Bernhardt
Nov 9, 2012·Analytical Chemistry·Palraj KalimuthuPaul V Bernhardt
Jul 13, 2004·Biomacromolecules·Christine M MooreShelley D Minteer