Miniaturized dispersive liquid-liquid microextraction and MALDI MS using ionic liquid matrices for the detection of bacterial communication molecules and virulence factors

Analytical and Bioanalytical Chemistry
Jan LeipertAndreas Tholey

Abstract

The identification and quantification of molecules involved in bacterial communication are major prerequisites for the understanding of interspecies interactions at the molecular level. We developed a procedure allowing the determination of 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS) and the virulence factor pyocyanin (PYO) formed by the Gram-negative bacterium Pseudomonas aeruginosa. The method is based on dispersive liquid-liquid microextraction from small supernatant volumes (below 10 μL) followed by quantitative matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). The use of ionic liquid matrix led to a lowered limit of detection for pyocyanin and, due to suppression of matrix background signals, easy to interpret mass spectra compared to crystalline matrices. Using an isotope-labeled pyocyanin standard synthesized in small-scale synthesis, quantitative analysis spanning approximately one order of magnitude (0.5 to 250 fmol) was feasible. The method was successfully applied to the detection of the signaling molecules PQS and HHQ in cultures of P. aeruginosa strains isolated from sputum of cystic fibrosis patients and allowed a highly sensitive quantification of PYO from...Continue Reading

References

Sep 1, 1986·European Journal of Biochemistry·D WatsonG W Taylor
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·E C PesciB H Iglewski
Sep 11, 2002·Analytical and Bioanalytical Chemistry·Lucinda H Cohen, Arkady I Gusev
Sep 25, 2002·Journal of Mass Spectrometry : JMS·Andreas TholeyElmar Heinzle
Mar 17, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yunxia Q O'MalleyBradley E Britigan
May 18, 2004·Journal of the American Society for Mass Spectrometry·François LépineLaurence G Rahme
Jun 26, 2004·Biotechnology Letters·Ditte BungertAndreas Tholey
May 3, 2005·Journal of the American Society for Mass Spectrometry·Ying L LiFong-Fu Hsu
Aug 17, 2005·Current Pharmaceutical Design·W Pusch, M Kostrzewa
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Christopher M Waters, Bonnie L Bassler
Nov 22, 2005·Emergency Medicine Journal : EMJ·J C R Rippey, M I Stallwood
Feb 2, 2006·Analytical Chemistry·R LemaireI Fournier
Feb 18, 2006·International Journal of Medical Microbiology : IJMM·Stephen P DiggleMiguel Cámara
Feb 25, 2006·Science·Andrew Camilli, Bonnie L Bassler
Feb 1, 2008·Pulmonary Pharmacology & Therapeutics·Sophie Moreau-MarquisGeorge A O'Toole
Aug 12, 2009·Journal of the American Society for Mass Spectrometry·Jeffrey A Crank, Daniel W Armstrong
Aug 29, 2009·Rapid Communications in Mass Spectrometry : RCM·Katerina SládkováJosef Havel
Sep 30, 2009·Journal of Mass Spectrometry : JMS·Roman Ullmer, Andreas M Rizzi
Nov 7, 2009·The American Journal of Pathology·Charles C CaldwellGee W Lau
Aug 27, 2010·FEMS Microbiology Reviews·Stephan HeebMiguel Cámara
Apr 7, 2012·Nature Medicine·Taylor Sitarik Cohen, Alice Prince
Aug 7, 2012·American Journal of Respiratory Cell and Molecular Biology·Ryan C HunterDianne K Newman
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Wilna J MoreePieter C Dorrestein
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Breah LaSarre, Michael J Federle
Feb 8, 2014·Virulence·José L Martínez
May 14, 2014·Methods in Molecular Biology·Catharine A OrtoriDavid A Barrett
May 30, 2015·The European Respiratory Journal·Helen L BarrAndrew W Fogarty
Aug 16, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kamlesh Shrivas, Kavita Tapadia
Dec 2, 2015·The ISME Journal·Robert A QuinnForest L Rohwer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.