Minichromosome maintenance protein 2 correlates with the malignant status and regulates proliferation and cell cycle in lung squamous cell carcinoma

OncoTargets and Therapy
Wei WuFengzhu Li

Abstract

Minichromosome maintenance protein 2 (MCM2), which is a member of MCM family, has been found to be a relevant marker for progression and prognosis in a variety of human cancers. Due to lack of effective therapeutic target in lung squamous cell carcinoma (LUSC) patients, the aim of our study was to screen and identify biomarkers which are associated to clinicopathological characteristics including prognosis in LUSC patients. The expression status of MCM2 in lung cancer was analyzed using the publicly available Gene Expression Omnibus databases (GSE3268 and GSE10245). The mRNA and protein expression of MCM2 in lung cancer tissues and cell lines was detected by quantitative real-time PCR and Western blot, and the association between MCM2 expression and clinicopathological factors was analyzed by immunohistochemistry. The loss-of-function study of MCM2 was conducted in LUSC cell lines. In our study, we found MCM2 expression was increased in LUSC tissues compared with paired adjacent normal lung tissues or lung adenocarcinoma tissues through analyzing microarray data sets (GSE3268 and GSE10245), which confirmed that MCM2 mRNA and protein were overexpressed in LUSC tissues and cell lines. Meanwhile, we analyzed the association betwee...Continue Reading

Citations

Feb 25, 2020·Analytical Cellular Pathology (Amsterdam)·Si YuYang Chen
Jul 10, 2021·Frontiers in Molecular Biosciences·Jian ZhouGen Wu
Sep 4, 2021·Journal of Oncology·Xinyu LiuChunying Shi
Feb 8, 2022·Diagnostic Pathology·Dorsay SadeghianBehnaz Jahanbin

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Datasets Mentioned

BETA
GSE10245
GSE3268

Methods Mentioned

BETA
PCR
transfection
flow cytometry

Software Mentioned

SPSS
Quantity One
GraphPad Prism

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