Minicollagen-15, a novel minicollagen isolated from Hydra, forms tubule structures in nematocysts

Journal of Molecular Biology
Patrizia AdamczykSuat Ozbek

Abstract

Minicollagens constitute a family of unusually short collagen molecules isolated from cnidarians. They are restricted to the nematocyst, a cylindrical explosive organelle serving in defense and capture of prey. The nematocyst capsule contains a long tubule inside of its matrix, which is expelled and everted during an ultrafast discharge process. Here, we report the cloning and characterization of a novel minicollagen in Hydra, designated minicollagen-15 (NCol-15). NCol-15, like NCol-3 and NCol-4, shows deviations from the canonical cysteine pattern in its terminal cysteine-rich domains (CRDs). Minicollagens share common domain architectures with a central collagen sequence flanked by polyproline stretches and short N- and C-terminal CRDs. The CRDs are involved in the formation of a highly resistant cysteine network, which constitutes the basic structure of the nematocyst capsule. Unlike NCol-1, which is part of the capsule wall, NCol-15 is localized to tubules, arguing for a functional differentiation of minicollagens within the nematocyst architecture. NMR analysis of the altered C-terminal CRD of NCol-15 showed a novel disulfide-linked structure within the cysteine-containing region exhibiting similar folding kinetics and sta...Continue Reading

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Citations

Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jung Shan HwangTakashi Gojobori
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Anna-Marei BoehmThomas C G Bosch
Jun 12, 2010·The Journal of Biological Chemistry·Patrizia AdamczykSuat Ozbek
Sep 3, 2010·Proceedings. Biological Sciences·Jason W HollandChris J Secombes
Jan 24, 2009·Genome Biology·Sabine MildeThomas C G Bosch
Aug 11, 2011·PloS One·Claudia ZenkertSuat Özbek
Dec 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·E Sally ChangPaulyn Cartwright
Sep 30, 2014·BMC Evolutionary Biology·Erez ShpirerDorothée Huchon
Mar 17, 2009·Toxicon : Official Journal of the International Society on Toxinology·Suat OzbekThomas W Holstein
Aug 5, 2008·Trends in Genetics : TIG·Charles N DavidThomas W Holstein
Nov 5, 2010·Trends in Genetics : TIG·Robert E SteeleUlrich Technau
Sep 19, 2009·Seminars in Cell & Developmental Biology·Hiroshi WatanabeThomas W Holstein
Sep 14, 2018·BMC Evolutionary Biology·Erez ShpirerDorothée Huchon
Dec 15, 2019·Scientific Reports·Theresa BenteleMotomu Tanaka
Feb 11, 2020·Frontiers in Chemistry·Sanaz FarajollahiRajesh R Naik
Jul 25, 2020·International Journal for Parasitology·Benjamin AmericusStephen D Atkinson
Aug 5, 2021·Journal of Cell Science·Jasmin MoneerAngelika Böttger

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