Minimal agreement between basophil activation test and immunoassay in diagnosis of penicillin allergy.

Allergologia et immunopathologia
B LeecyousN Abdullah

Abstract

Basophil activation test (BAT) and immunoassays are the most widely used in vitro tests to diagnose IgE-mediated allergic reactions to penicillin. However, studies to determine if one test is interdependent from another are limited. The present study aimed to measure the agreement between BAT and immunoassay in diagnosis of penicillin allergy. BAT was performed using penicillin G (Pen G), penicillin V (Pen V), penicilloyl-polylysine (PPL), minor determinant mix (MDM), amoxicillin (Amx) and ampicillin (Amp) in 25 patients. Immunoassay of total IgE (tIgE) and specific IgE (sIgE) antibodies to Pen G, Pen V, Amx and Amp were quantified. Skin prick test (SPT) using PPL-MDM, Amx, Amp and Clavulanic acid were also performed. Minimal agreement was observed between BAT and immunoassay (k=0.25). Of two BAT-positive patients, one patient is positive to Amx (59.27%, SI=59) and Amp (82.32%, SI=82) but sIgE-negative to all drug tested. This patient is also SPT-positive to both drugs. Another patient is BAT-positive to Pen G (10.18%, SI=40), Pen V (25.07%, SI=100) and Amp (19.52%, SI=79). In sIgE immunoassay, four patients were sIgE-positive to at least one of the drugs tested. The sIgE level of three patients was between low and moderate and...Continue Reading

References

Sep 27, 2003·Allergy·M J TorresUNKNOWN EAACI Interest Group on Drug Hypersensitivity
Aug 19, 2004·The Journal of Allergy and Clinical Immunology·Robert G Hamilton, N Franklin Adkinson
Jun 18, 2011·The Journal of Pharmacology and Experimental Therapeutics·Xiaoli MengB Kevin Park
Jan 24, 2012·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Faisal M KhanJan Storek
Dec 29, 2012·The Journal of Allergy and Clinical Immunology·S G O JohanssonAnna Nopp
Mar 19, 2016·Allergy·C MayorgaUNKNOWN In vitro tests for Drug Allergy Task Force of EAACI Drug Interest Group
Jun 8, 2017·International Journal of Molecular Sciences·Cristobalina MayorgaMaria J Torres
Jul 4, 2017·JAMA : the Journal of the American Medical Association·Jason A TrubianoElizabeth Jane Phillips
Aug 29, 2017·Annals of Medicine·Angèle SoriaJean-Eric Autegarden
Oct 2, 2017·The Journal of Allergy and Clinical Immunology. in Practice·María SalasMaría José Torres
Jul 26, 2018·International Archives of Allergy and Immunology·Paolo MarracciniDario Consonni
Sep 25, 2018·The Journal of Allergy and Clinical Immunology. in Practice·Maria J TorresUNKNOWN AAAAI/WAO 2018 Symposium Penicillin and Cephalosporin Allergy Testing Working Group

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