Abstract
Basophil activation test (BAT) and immunoassays are the most widely used in vitro tests to diagnose IgE-mediated allergic reactions to penicillin. However, studies to determine if one test is interdependent from another are limited. The present study aimed to measure the agreement between BAT and immunoassay in diagnosis of penicillin allergy. BAT was performed using penicillin G (Pen G), penicillin V (Pen V), penicilloyl-polylysine (PPL), minor determinant mix (MDM), amoxicillin (Amx) and ampicillin (Amp) in 25 patients. Immunoassay of total IgE (tIgE) and specific IgE (sIgE) antibodies to Pen G, Pen V, Amx and Amp were quantified. Skin prick test (SPT) using PPL-MDM, Amx, Amp and Clavulanic acid were also performed. Minimal agreement was observed between BAT and immunoassay (k=0.25). Of two BAT-positive patients, one patient is positive to Amx (59.27%, SI=59) and Amp (82.32%, SI=82) but sIgE-negative to all drug tested. This patient is also SPT-positive to both drugs. Another patient is BAT-positive to Pen G (10.18%, SI=40), Pen V (25.07%, SI=100) and Amp (19.52%, SI=79). In sIgE immunoassay, four patients were sIgE-positive to at least one of the drugs tested. The sIgE level of three patients was between low and moderate and...Continue Reading
References
Oct 3, 1999·Allergy·P DemolyW J Pichler
Sep 27, 2003·Allergy·M J TorresUNKNOWN EAACI Interest Group on Drug Hypersensitivity
Aug 19, 2004·The Journal of Allergy and Clinical Immunology·Robert G Hamilton, N Franklin Adkinson
Jan 31, 2009·Allergy·T D FernándezM Blanca
Jul 9, 2010·The Medical Clinics of North America·Maria J Torres, Miguel Blanca
Jun 18, 2011·The Journal of Pharmacology and Experimental Therapeutics·Xiaoli MengB Kevin Park
Jan 24, 2012·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Faisal M KhanJan Storek
Dec 29, 2012·The Journal of Allergy and Clinical Immunology·S G O JohanssonAnna Nopp
Apr 17, 2015·PloS One·Alessandra VultaggioAndrea Matucci
Jan 1, 2014·Clinical and Translational Allergy·Janni HjortlundCarsten Bindslev-Jensen
Jul 23, 2015·Allergy·H J HoffmannE F Knol
Mar 19, 2016·Allergy·C MayorgaUNKNOWN In vitro tests for Drug Allergy Task Force of EAACI Drug Interest Group
Mar 16, 2017·Clinical and Translational Allergy·M J TorresP Bonadonna
Jun 8, 2017·International Journal of Molecular Sciences·Cristobalina MayorgaMaria J Torres
Jul 4, 2017·JAMA : the Journal of the American Medical Association·Jason A TrubianoElizabeth Jane Phillips
Aug 29, 2017·Annals of Medicine·Angèle SoriaJean-Eric Autegarden
Oct 2, 2017·The Journal of Allergy and Clinical Immunology. in Practice·María SalasMaría José Torres
Jul 26, 2018·International Archives of Allergy and Immunology·Paolo MarracciniDario Consonni
Sep 25, 2018·The Journal of Allergy and Clinical Immunology. in Practice·Maria J TorresUNKNOWN AAAAI/WAO 2018 Symposium Penicillin and Cephalosporin Allergy Testing Working Group