Minimal change-like glomerular alterations induced by a human plasma factor

Nephron
P K CheungW W Bakker

Abstract

Circulating factors, including the plasma protease (100 KF) described previously, have been suspected to play a role in the pathogenesis of minimal change disease (MCD) for several decades. This factor was able to induce MCD-like alterations in kidney tissue in vitro, i.e. impairment of glomerular polyanion (GPA), as well as glomerular ecto-ATPase. We conducted permeability studies using alternate perfusion of the rat kidney ex vivo according to standard techniques. Either native 100 KF (n = 7) or control factor (n = 7) perfusion, followed by perfusion with diluted rat serum was carried out, while urine samples were collected by ureter cannulation. Total urinary protein (by spectrophotometry) as well as IgG (by ELISA) and albumin (by rocket electrophoresis) were measured. Sections of perfused kidneys were stained (immuno-) histochemically for GPA and glomerular ecto-ATPase, and the stainability was quantified using image analysis and expressed as arbitrary units. The results show significantly increased protein leakage after perfusion of 100 KF versus control factor (150.0 +/- 48.9 vs. 33.2 +/- 7.7 micrograms/min, p < or = 0.01), while the IgG/albumin ratio has decreased (12.0 +/- 9.4 vs 26.9 +/- 14.4%, p < or = 0.01). Plasma p...Continue Reading

Citations

Nov 10, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Caroline Hervé, Jacques Dantal
Jun 18, 2004·Kidney International·Maria del Carmen Avila-CasadoJaime Herrera-Acosta
Jul 15, 2005·Kidney International·Winston W BakkerJola J Kapojos
Aug 30, 2008·Journal of the American Society of Nephrology : JASN·Rachel LennonMoin A Saleem
Nov 25, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Rutger J MaasJack F Wetzels
Mar 20, 2012·Néphrologie & thérapeutique·Dil SahaliPhilippe Lang
Jul 8, 2016·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Gabriel Cara-FuentesEduardo H Garin
May 19, 2012·Reproductive Sciences·Winston W BakkerMarijke M Faas
May 1, 2007·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Winston W BakkerMarijke M Faas
Dec 14, 2012·American Journal of Physiology. Renal Physiology·Wilhelm KrizKevin V Lemley
Jan 23, 2003·Journal of the American Society of Nephrology : JASN·Richard J Glassock
Aug 14, 1999·Journal of the American Society of Nephrology : JASN·P K CheungW W Bakker
Jul 25, 2021·Journal of Clinical Medicine·Agnieszka Pukajło-Marczyk, Danuta Zwolińska

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