Minimal effect of acute experimental hepatitis induced by lipopolysaccharide/D-galactosamine on biotransformation in rats

Biochemical Pharmacology
Omar H el-DJ B Watkins

Abstract

When administered with D-galactosamine, lipopolysaccharide endotoxins produce a good experimental animal model of hepatitis. This galactosamine plus endotoxin model has been used widely, but the acute effect of this fixed combination of two chemicals on hepatic and extrahepatic biotransformation has not been determined. Therefore, either 2 or 4 hr after a single intraperitoneal dose of 300 mg/kg galactosamine plus 30 micrograms/kg lipopolysaccharide was administered, serum, liver, kidney, intestine, and spleen were collected. Serum enzymes (alanine and aspartate aminotransferases, sorbitol dehydrogenase, and gamma-glutamyltranspeptidase) were elevated dramatically 2 and 4 hr after treatment. Cytochrome P450 monooxygenase activity toward benzo-[a]pyrene was increased in kidney 4 hr after treatment, whereas dealkylation of 7-methoxycoumarin or 7-ethoxyresorufin was unchanged in any tissue at either time point. An increase in UDP-glucuronosyltransferase activity toward 4-methylumbelliferone and 4-hydroxybiphenyl was noted in the intestine. Conjugation of 1-chloro-2,4-dinitrobenzene with glutathione was increased in intestine and spleen 2 hr after treatment. gamma-Glutamyltranspeptidase activity was unaltered in all tissues studied...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C GalanosW Reutter
Feb 1, 1976·Biochemical Pharmacology·F GorodischerS J Yaffe
Sep 1, 1976·Archives of Biochemistry and Biophysics·D M Bissell, L E Hammaker
Jan 1, 1990·Methods in Enzymology·A Wendel
Nov 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J P Nolan
Jun 15, 1987·Biochemical Pharmacology·A Wendel, G Tiegs
Jul 31, 1972·Biochimica Et Biophysica Acta·N S KosowerE M Kosower
Mar 9, 1981·FEBS Letters·K AnjaneyuluW J Malaisse
Jan 1, 1981·Methods in Enzymology·A MeisterO W Griffith
Aug 1, 1984·Analytical Biochemistry·L C Mokrasch, E J Teschke
Feb 15, 1980·Biochemical Pharmacology·K W BockK L Platt
Jan 1, 1981·Methods in Enzymology·W H Habig, W B Jakoby
Nov 1, 1994·Gut·B SmedsrødA Geerts
Dec 1, 1994·The Journal of Clinical Investigation·S I ShedlofskyR A Blouin
Apr 1, 1993·The International Journal of Biochemistry·C García-AlfonsoJ López-Barea
Dec 1, 1960·The Journal of Experimental Medicine·T WIZNITZERJ FINE

❮ Previous
Next ❯

Citations

Jun 1, 2005·Journal of Ethnopharmacology·Vilwanathan RavikumarThiruvengadam Devaki
Apr 18, 2013·Asian Pacific Journal of Tropical Biomedicine·Sheik Abdulazeez Sheriff, Thiruvengadam Devaki
Jul 5, 2005·Expert Opinion on Investigational Drugs·J WuM A Zern
Sep 24, 2010·Pharmaceutical Biology·Hussain ZeashanChandana Venkateswara Rao
Sep 14, 2013·Pharmaceutical Biology·Sheriff Sheik Abdulazeez, Devaki Thiruvengadam
Jul 8, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Haiping HaoGuangji Wang
Jan 16, 1999·Journal of Biochemical and Molecular Toxicology·A M WatsonR A Blouin
Aug 2, 2017·Journal of Proteome Research·Sheik Abdulazeez SheriffHoracio Pérez-Sánchez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.