PMID: 7541138Jun 20, 1995Paper

Minimal epitopes expressed in a recombinant polyepitope protein are processed and presented to CD8+ cytotoxic T cells: implications for vaccine design

Proceedings of the National Academy of Sciences of the United States of America
S A ThomsonA Suhrbier

Abstract

The epitopes recognized by CD8+ cytotoxic T lymphocytes (CTL) are generated from cytosolic proteins by proteolytic processing. The nature of the influences exerted by the sequences flanking CTL epitopes on these processing events remains controversial. Here we show that each epitope within an artificial polyepitope protein containing nine minimal CD8+ CTL epitopes in sequence was processed and presented to appropriate CTL clones. Natural flanking sequences were thus not required to direct class I proteolytic processing. In addition, unnatural flanking sequences containing other CTL epitopes did not interfere with processing. The ability of every CTL epitope to be effectively processed from a protein containing only CTL epitopes is likely to find application in the construction of recombinant polyepitope CTL vaccines.

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Citations

Apr 1, 1997·Reviews in Medical Virology·F Radecke, M A Billeter
May 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G J Waine, D P McManus
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Aug 1, 1997·Immunology and Cell Biology·A Suhrbier
Aug 4, 2010·Immunology and Cell Biology·Paweena RattanasenaAndreas Suhrbier
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Jul 10, 2010·Journal of Biomedicine & Biotechnology·Roberto Bei, Antonio Scardino

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