Minimal Escherichia coli cell for the most efficient production of ethanol from hexoses and pentoses.

Applied and Environmental Microbiology
Cong T TrinhFriedrich Srienc

Abstract

To obtain an efficient ethanologenic Escherichia coli strain, we reduced the functional space of the central metabolic network, with eight gene knockout mutations, from over 15,000 pathway possibilities to 6 pathway options that support cell function. The remaining pathways, identified by elementary mode analysis, consist of four pathways with non-growth-associated conversion of pentoses and hexoses into ethanol at theoretical yields and two pathways with tight coupling of anaerobic cell growth with ethanol formation at high yields. Elimination of three additional genes resulted in a strain that selectively grows only on pentoses, even in the presence of glucose, with a high ethanol yield. We showed that the ethanol yields of strains with minimized metabolic functionality closely matched the theoretical predictions. Remarkably, catabolite repression was completely absent during anaerobic growth, resulting in the simultaneous utilization of pentoses and hexoses for ethanol production.

References

Aug 1, 1989·Applied and Environmental Microbiology·F Alterthum, L O Ingram
Oct 20, 1995·Science·C M FraserJ C Venter
Apr 1, 1995·Applied Microbiology and Biotechnology·S E LindsayL O Ingram
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·A R Mushegian, E V Koonin
May 9, 1998·Applied and Environmental Microbiology·N W HoA P Brainard
Jun 5, 1998·Journal of Industrial Microbiology & Biotechnology·L P YomanoL O Ingram
Aug 24, 1999·Journal of Industrial Microbiology & Biotechnology·G J DumsdayN B Pamment
Oct 9, 1999·Biotechnology Progress·L O IngramS Zhou
Oct 9, 1999·Biotechnology Progress·R J BothastB S Dien
Dec 11, 1999·Science·C A HutchisonJ C Venter
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jun 13, 2000·Applied Biochemistry and Biotechnology·B S DienR J Bothast
Aug 14, 2001·Applied Microbiology and Biotechnology·N N NicholsR J Bothast
Nov 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Daniel SegrèGeorge M Church
Nov 15, 2002·Nature·Jörg StellingErnst Dieter Gilles
Oct 13, 1956·Nature·B MAGASANIK, F C NEIDHARDT
Nov 5, 2003·Biotechnology and Bioengineering·Anthony P BurgardCostas D Maranas
Jan 6, 2004·Biotechnology and Bioengineering·Ross Carlson, Friedrich Srienc
Sep 17, 2004·Cell·Jörg StellingJohn Doyle
Dec 21, 2004·Nucleic Acids Research·Ingrid M KeselerPeter D Karp
Jun 18, 2005·Biotechnology and Bioengineering·Stephen S FongBernhard O Palsson
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·John I GlassJ Craig Venter
Jan 28, 2006·Science·Arthur J RagauskasTimothy Tschaplinski
Apr 29, 2006·Science·György PósfaiFrederick R Blattner
May 30, 2006·Bioinformatics·Axel von Kamp, Stefan Schuster
Aug 23, 2006·Molecular Systems Biology·Anthony C Forster, George M Church
Sep 26, 2006·Metabolic Engineering·Cong T TrinhFriedrich Srienc
Aug 1, 2007·Advances in Biochemical Engineering/biotechnology·L R JarboeL O Ingram
Nov 1, 2005·EcoSal Plus·Christoph Mayer, Winfried Boos

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Citations

Nov 19, 2008·Applied Microbiology and Biotechnology·Cong T TrinhFriedrich Srienc
Jun 17, 2009·Applied Microbiology and Biotechnology·Miho SasakiHideaki Yukawa
Feb 10, 2010·Applied Microbiology and Biotechnology·James M Clomburg, Ramon Gonzalez
Jan 9, 2013·Applied Microbiology and Biotechnology·Petra ZajkoskaMichal Rosenberg
Mar 1, 2012·Journal of Industrial Microbiology & Biotechnology·Ryan ManowShengde Zhou
Mar 12, 2013·Bio Systems·Dimitrije Jevremović, Daniel Boley
Jul 16, 2013·Journal of Agricultural and Food Chemistry·Chung-Jen ChiangYun-Peng Chao
Mar 1, 2012·Nature Reviews. Microbiology·Nathan E LewisBernhard O Palsson
Feb 23, 2010·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Dimitrije JevremovicDaniel Boley
Aug 22, 2012·Briefings in Functional Genomics·Cecilia KleinMatteo Brilli
Dec 23, 2011·Bioinformatics·Kathrin BallersteinUtz-Uwe Haus
Sep 11, 2012·Bioinformatics·Daniel MachadoIsabel Rocha
May 4, 2013·Plant Physiology·Mark G PoolmanDavid A Fell
Jun 7, 2011·Applied and Environmental Microbiology·Cong T TrinhDouglas S Clark
Sep 8, 2009·Applied and Environmental Microbiology·Cong T Trinh, Friedrich Srienc
Jul 26, 2011·Applied and Environmental Microbiology·Sarah HufferDouglas S Clark
Aug 10, 2010·Applied and Environmental Microbiology·Shital A TripathiNicky C Caiazza
Jan 1, 2010·Journal of Biomedicine & Biotechnology·Jennifer L ReedJamey D Young
Sep 10, 2011·Microbial Cell Factories·Fumio MatsudaAkihiko Kondo
Aug 18, 2012·Microbial Cell Factories·Sonja Billerbeck, Sven Panke
Sep 26, 2013·Microbial Cell Factories·Evamaria GruchattkaOliver Kayser
Aug 18, 2012·BMC Systems Biology·Christian Jungreuthmayer, Jürgen Zanghellini
Nov 23, 2013·BMC Systems Biology·Mona YousofshahiSoha Hassoun
Dec 15, 2012·Genome Biology·Christopher J Tervo, Jennifer L Reed
Apr 27, 2010·PLoS Computational Biology·Sridhar RanganathanCostas D Maranas
Jan 7, 2014·PLoS Computational Biology·Axel von Kamp, Steffen Klamt
Mar 29, 2014·PloS One·David E RuckerbauerJürgen Zanghellini
Nov 7, 2013·BMC Bioinformatics·Christian JungreuthmayerJürgen Zanghellini
May 15, 2012·Annual Review of Biomedical Engineering·Allen A Cheng, Timothy K Lu
Sep 15, 2014·Bio Systems·Daniel MachadoIsabel Rocha
Dec 20, 2015·Bioinformatics·Jan Bert van Klinken, Ko Willems van Dijk
Jan 19, 2016·Frontiers in Physiology·Cheng Zhang, Qiang Hua
Feb 24, 2016·Microbes and Infection·Sophia Häfner
Nov 28, 2012·Trends in Biotechnology·James M Clomburg, Ramon Gonzalez
Sep 10, 2011·Metabolic Engineering·Pornkamol Unrean, Friedrich Srienc

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