Minimal Residual Disease Detection by Droplet Digital PCR in Multiple Myeloma, Mantle Cell Lymphoma, and Follicular Lymphoma: A Comparison with Real-Time PCR

The Journal of Molecular Diagnostics : JMD
D DrandiM Ladetto

Abstract

Real-time quantitative PCR (qPCR) is a well-established tool for minimal residual disease (MRD) detection in mature lymphoid malignancies. Despite remarkable sensitivity and specificity, qPCR has some limitations, particularly in the need for a reference standard curve, based on target serial dilutions. In this study, we established droplet digital PCR (ddPCR) for MRD monitoring in multiple myeloma, mantle cell lymphoma, and follicular lymphoma and compared it head-to-head with qPCR. We observed that ddPCR has sensitivity, accuracy, and reproducibility comparable with qPCR. We then compared the two approaches in 69 patients with a documented molecular marker at diagnosis (18 multiple myelomas, 21 mantle cell lymphomas assessed with the immunoglobulin gene rearrangement, and 30 follicular lymphomas with the use of the BCL2/immunoglobulin gene major breakpoint region rearrangement). ddPCR was successful in 100% of cases, whereas qPCR failed to provide a reliable standard curve in three patients. Overall, 222 of 225 samples were evaluable by both methods. The comparison highlighted a good concordance (r = 0.94, P < 0.0001) with 189 of 222 samples (85.1%; 95% CI, 80.4%-89.8%) being fully concordant. We found that ddPCR is a reliabl...Continue Reading

References

Jun 28, 2000·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·M LadettoJ G Gribben
Feb 9, 2007·Leukemia·V H J van der VeldenUNKNOWN European Study Group on MRD detection in ALL (ESG-MRD-ALL)
Aug 2, 2008·Blood·Bruno PaivaUNKNOWN GEM (Grupo Español de MM)/PETHEMA (Programa para el Estudio de la Terapéutica en Hemopatías Malignas) Cooperative Study Grou
Aug 5, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Niels S AndersenChristian H Geisler
Feb 2, 2010·Methods : a Companion to Methods in Enzymology·Amelia L MarkeyPhilip J R Day
Mar 24, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Marco LadettoAntonio Palumbo
Feb 5, 2011·Blood·S Vincent RajkumarUNKNOWN International Myeloma Workshop Consensus Panel 1
Jul 13, 2011·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Mark KorthalsRoland Fenk
Nov 1, 2011·Analytical Chemistry·Benjamin J HindsonBill W Colston
Nov 30, 2011·Analytical Chemistry·Leonardo B PinheiroKerry R Emslie
Dec 1, 2011·Blood·Bruno PaivaUNKNOWN PETHEMA/GEM (Programa para el Estudio de la Terapéutica en Hemopatías Malignas/Grupo Español de Mieloma) Cooperative Study G
Feb 1, 2012·Seminars in Oncology·Monika BrüggemannMichael Kneba
Dec 12, 2012·Journal of Clinical Microbiology·R T HaydenA M Caliendo
Apr 11, 2013·PloS One·Matthew C StrainDouglas D Richman
Jun 5, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Andy C RawstronRoger G Owen
Sep 3, 2013·Nature Methods·Christopher M HindsonMuneesh Tewari
Sep 5, 2013·Clinical Chemistry·Tanis C DingleKeith R Jerome
Oct 9, 2013·Clinical Chemistry·Jim F Huggett, Alexandra Whale
Nov 5, 2013·Analytical Chemistry·Geoffrey P McDermottAdam J Lowe
Jan 7, 2014·The Journal of Molecular Diagnostics : JMD·Lawrence J JenningsLoren Joseph
Oct 24, 2014·Clinical Chemistry·Jim F HuggettCarole A Foy

❮ Previous
Next ❯

Citations

Apr 5, 2016·International Journal of Molecular Sciences·Susana Olmedillas LópezDamián García-Olmo
Jul 14, 2016·Expert Review of Hematology·E ZamagniM Cavo
Mar 8, 2017·The Journal of Molecular Diagnostics : JMD·Claudia BrunettiFrancesco Albano
May 20, 2017·Expert Review of Molecular Diagnostics·Marta Lionetti, Antonino Neri
Jul 4, 2017·European Journal of Cancer Care·V InnaoC Musolino
Jun 27, 2017·Cancer Treatment Reviews·Peter MartinMartin Dreyling
Dec 22, 2017·British Journal of Haematology·Yinlei BaiChor Sang Chim
Oct 12, 2017·Leukemia & Lymphoma·Uday Yanamandra, Shaji K Kumar
Jul 5, 2018·Expert Review of Anticancer Therapy·Alessandro PulsoniRobin Foà
Feb 28, 2019·Current Hematologic Malignancy Reports·Jennifer Crombie, Philippe Armand
Jun 14, 2018·Blood Advances·Farhad RavandiSylvie D Freeman
Feb 6, 2020·Oncotarget·Ayad M Al-KatibAli M Gabali
Nov 12, 2016·Oncotarget·Angela MinerviniFrancesco Albano
Aug 22, 2017·The Journal of Clinical Investigation·Brian ParkinSami N Malek
May 10, 2017·Molecular Diagnosis & Therapy·Susana Olmedillas-LópezDamián García-Olmo
Aug 24, 2018·Current Hematologic Malignancy Reports·Justin M Darrah, Alex F Herrera
Sep 21, 2018·Journal of Clinical Medicine·Irene DogliottiSimone Ferrero
Feb 21, 2019·British Journal of Haematology·Paul J GalardyRodney R Miles
Sep 27, 2019·Frontiers in Oncology·Ilaria Del GiudiceRobin Foà
Dec 4, 2016·Hematology·Gert Ossenkoppele, Gerrit Jan Schuurhuis
Nov 8, 2018·Current Treatment Options in Oncology·Daniele GrimaldiMarco Ladetto
Aug 18, 2018·Bone Marrow Transplantation·Marco LadettoPeter Bader
Sep 12, 2017·Expert Review of Molecular Diagnostics·Vittorio NunesA Biondi
Aug 27, 2019·Frontiers in Oncology·Irene Della StarzaRobin Foà
Jun 20, 2020·JCI Insight·Eileen Y HuJohn C Byrd
Sep 26, 2020·Journal of Hematology & Oncology·Dayoung JungMichael Wang
May 6, 2020·International Journal of Molecular Sciences·Nicoletta CoccaroFrancesco Albano
Nov 6, 2020·Journal of Clinical and Experimental Hematopathology : JCEH·Takayoshi UchiyamaSadao Aoki
Jun 5, 2020·The Cancer Journal·James P Solomon, Maria E Arcila

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.