PMID: 9557683Apr 29, 1998Paper

Minimal template requirements for initiation of minus-strand synthesis in vitro by the RNA-dependent RNA polymerase of turnip yellow mosaic virus

Journal of Virology
B A DeimanC W Pleij

Abstract

From mutational analysis of the 3'-terminal hairpin of turnip yellow mosaic virus (TYMV) RNA and use of nonstructured C-rich RNA templates, we conclude that the main determinant in the tRNA-like structure of TYMV RNA for initiation of minus-strand synthesis by the viral RNA-dependent RNA polymerase (RdRp) is the non-base-paired 3' ACC(A) end. Base pairing of this 3' end reduces the transcription efficiency drastically, and deletion of only the 3'-terminal A residue results in a fivefold drop in efficiency. The two C residues of the 3' ACCA end are required for efficient transcription, as shown by substitution mutations. However, the 5' A residue is not specifically involved in initiation of transcription, as shown by substitution mutations. Furthermore, the hairpin stem and loop upstream of the 3' ACCA end also do not interact with the RdRp in a base-specific way. However, for efficient transcription, the hairpin stem should be at least five bp in length, while the calculated deltaG value should be less than -10.5 kcal/mol. Unexpectedly, the use of nonstructured C-rich RNA templates showed that the RdRp can start internally on an NCCN or NUCN sequence. Therefore, a possible function of the tRNA-like structure of TYMV RNA may be...Continue Reading

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Citations

Apr 30, 2003·Journal of Virology·K SivakumaranC Cheng Kao
Nov 10, 2001·Annual Review of Phytopathology·Theo W. Dreher
Oct 24, 2008·Phytopathology·J J BernalE Rodríguez-Cerezo
Jul 17, 2007·Nature Reviews. Microbiology·Ian BrierleyRobert J C Gilbert
Apr 24, 2004·The Journal of General Virology·Alberdina A van DijkDennis H Bamford
Mar 30, 2005·Journal of Virology·Richard W Hardy, Charles M Rice
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Theo W Dreher
Apr 30, 2017·Frontiers in Microbiology·I-Hsuan ChenChing-Hsiu Tsai
Mar 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Anna V SherwoodTina M Henkin
Sep 5, 2001·Virology·C C KaoD J Ecker
Apr 4, 2021·RNA·Conner J LangebergJeffrey S Kieft

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