Minimalist design of water-soluble cross-beta architecture.

Proceedings of the National Academy of Sciences of the United States of America
Matthew BiancalanaS Koide

Abstract

Demonstrated successes of protein design and engineering suggest significant potential to produce diverse protein architectures and assemblies beyond those found in nature. Here, we describe a new class of synthetic protein architecture through the successful design and atomic structures of water-soluble cross-beta proteins. The cross-beta motif is formed from the lamination of successive beta-sheet layers, and it is abundantly observed in the core of insoluble amyloid fibrils associated with protein-misfolding diseases. Despite its prominence, cross-beta has been designed only in the context of insoluble aggregates of peptides or proteins. Cross-beta's recalcitrance to protein engineering and conspicuous absence among the known atomic structures of natural proteins thus makes it a challenging target for design in a water-soluble form. Through comparative analysis of the cross-beta structures of fibril-forming peptides, we identified rows of hydrophobic residues ("ladders") running across beta-strands of each beta-sheet layer as a minimal component of the cross-beta motif. Grafting a single ladder of hydrophobic residues designed from the Alzheimer's amyloid-beta peptide onto a large beta-sheet protein formed a dimeric protein ...Continue Reading

References

Apr 5, 1973·Journal of Molecular Biology·C Chothia
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·S SteinbacherR Huber
Nov 14, 1997·Journal of Molecular Biology·M SundeC C Blake
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·D E OtzenM Oliveberg
Aug 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Riechmann, G Winter
Mar 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jane S Richardson, David C Richardson
Aug 7, 2002·Current Opinion in Structural Biology·Todd O Yeates, Jennifer E Padilla
Nov 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Manuela López De La PazLuis Serrano
Aug 15, 2003·Nature·Fabrizio ChitiChristopher M Dobson
Nov 25, 2003·Science·Brian KuhlmanDavid Baker
Dec 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Manuela López de la Paz, Luis Serrano
Mar 19, 2004·The EMBO Journal·Hye-Jeong YeoGabriel Waksman
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Richard A KammererMichel O Steinmetz
Jan 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·O Sumner MakinLouise C Serpell
Jun 10, 2005·Nature·Rebecca NelsonDavid Eisenberg
Nov 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alexandra Esteras-ChopoManuela López de la Paz
Nov 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Thorsten LührsRoland Riek
Nov 23, 2005·PLoS Biology·Douglas M FowlerJeffery W Kelly
Nov 24, 2005·The FEBS Journal·O Sumner Makin, Louise C Serpell
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Magdalena I IvanovaDavid Eisenberg
Mar 28, 2006·Current Opinion in Structural Biology·Rebecca Nelson, David Eisenberg
Apr 11, 2006·PLoS Biology·Philippe PeigneuxPierre Maquet
Jul 11, 2006·Protein Science : a Publication of the Protein Society·Koki MakabeShohei Koide
Jul 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Luciana EspositoLuigi Vitagliano
Oct 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Woojin Kim, Michael H Hecht
Nov 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Koki MakabeShohei Koide
Apr 5, 2007·Proteins·Chung-Jung TsaiRuth Nussinov
Apr 7, 2007·Trends in Biochemical Sciences·Douglas M FowlerJeffery W Kelly
May 1, 2007·Nature·Michael R SawayaDavid Eisenberg
Oct 5, 2007·Nature·Michele Vendruscolo, Christopher M Dobson
Oct 10, 2007·FEBS Letters·Clement AngkawidjajaShigenori Kanaya
Nov 24, 2007·Cellular and Molecular Life Sciences : CMLS·D Otzen, P H Nielsen
Jan 12, 2008·Science·Dirk GrueningerGeorg E Schulz
Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Wolfgang HoyerTorleif Härd
Apr 22, 2008·Journal of Molecular Biology·Shin-Wen LeeJerry C C Chan
Jun 17, 2008·Protein Science : a Publication of the Protein Society·Zhefeng Guo, David Eisenberg
Jun 17, 2008·Protein Science : a Publication of the Protein Society·Jed J W WiltziusDavid Eisenberg
Sep 3, 2008·Journal of Molecular Biology·Matthew BiancalanaShohei Koide

❮ Previous
Next ❯

Citations

Dec 7, 2010·Nature Structural & Molecular Biology·Cong LiuDavid Eisenberg
Sep 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Leslie S WolfeYong Xiong
Sep 29, 2011·BMC Structural Biology·Christopher P GarnhamPeter L Davies
Jan 18, 2018·Angewandte Chemie·Juan A VarelaSteven F Lee
Dec 21, 2012·Chemistry : a European Journal·Rouzbeh Afrasiabi, Heinz-Bernhard Kraatz
Oct 28, 2010·Chembiochem : a European Journal of Chemical Biology·Rafael Giraldo
May 17, 2019·Chembiochem : a European Journal of Chemical Biology·Shota ShigaKoki Makabe
Feb 26, 2016·Protein Science : a Publication of the Protein Society·Koli BasuPeter L Davies
Feb 3, 2011·Proteins·Volodymyr BabinCeleste Sagui
Sep 28, 2018·International Journal of Molecular Sciences·Dawid DułakIrena Roterman
Mar 23, 2021·Frontiers in Bioengineering and Biotechnology·Nicole BalascoAntonella Accardo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.