Minimally invasive system to reliably characterize ventricular electrophysiology from living donors.

Scientific Reports
Aida Oliván-VigueraEsther Pueyo

Abstract

Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratome-sliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verified viability. Optically mapped transmembrane potentials confirmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and β-adrenergic stimulati...Continue Reading

References

Oct 13, 1998·Journal of the American College of Cardiology·E DrouinF Charpentier
Dec 29, 2004·Cardiovascular Research·Patrizia CamellitiPeter Kohl
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Frank PillekampRuediger Koehling
Dec 3, 2005·Journal of Muscle Research and Cell Motility·C J Barclay
Jan 24, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Sergey F MironovArkady M Pertsov
May 17, 2007·Pflügers Archiv : European journal of physiology·Dirk W DonkerPaul G A Volders
Jun 19, 2007·Experimental Physiology·Massimiliano ZaniboniNicolò Salvarani
Jan 29, 2008·The Annals of Thoracic Surgery·Raimondo AscioneGianni D Angelini
Nov 17, 2009·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Alexandra BussekUrsula Ravens
Jan 23, 2010·Circulation Research·Alexey V GlukhovIgor R Efimov
Jul 12, 2011·Journal of Molecular and Cellular Cardiology·Patrizia CamellitiCesare M Terracciano
Oct 6, 2011·Cardiovascular Research·Matthias BrandenburgerAndreas Dendorfer
Nov 15, 2011·British Journal of Pharmacology·Herbert M HimmelErich Wettwer
May 23, 2012·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Rosanne VarkevisserMarc A Vos
Jan 18, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Ken WangChristian Bollensdorff
Feb 13, 2015·Circulation. Arrhythmia and Electrophysiology·Di LangIgor R Efimov
May 20, 2015·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Jorge G QuintanillaJulián Pérez-Villacastín
Dec 5, 2015·Circulation Research·Alexander R PintoMichelle D Tallquist
Jul 28, 2016·JACC. Basic to Translational Science·R Croft ThomasPaul C Simpson
Apr 27, 2017·Scientific Reports·Valentina CappelloMauro Gemmi
Oct 11, 2017·Cardiovascular Research·Filippo PerbelliniCesare M Terracciano
Dec 1, 2017·Nature Protocols·Samuel A WatsonFilippo Perbellini
Jun 13, 2018·Computers in Biology and Medicine·S PollnowG Lenis
Jul 2, 2018·Progress in Biophysics and Molecular Biology·Yun QiaoIgor R Efimov
Oct 26, 2018·Circulation. Arrhythmia and Electrophysiology·Virginie DubesOlivier Bernus
Jan 4, 2019·Nature Communications·Maksim KunitskiReinhard Dörner
Jan 11, 2019·Scientific Reports·Aldenor G SantosJailson B de Andrade
Feb 13, 2019·Cardiovascular Research·Peter LeeDavid Filgueiras-Rama
May 17, 2019·Nature Communications·Samuel A WatsonCesare M Terracciano
Jul 17, 2019·Circulation Research·Qinghui OuTamer M A Mohamed
May 12, 2020·PloS One·Dmitrii SmirnovIgor R Efimov

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
biopsy
coronary artery bypass
confocal microscopy

Software Mentioned

MATLAB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.