Minimally invasive technique for measuring transdermal glucose with a fluorescent biosensor

Analytical and Bioanalytical Chemistry
Sheniqua BrownLeah Tolosa

Abstract

There is a need for blood glucose monitoring techniques that eliminate the painful and invasive nature of current methods, while maintaining the reliability and accuracy of established medical technology. This research aims to ultimately address these shortcomings in critically ill pediatric patients. Presented in this work is an alternative, minimally invasive technique that uses microneedles (MN) for the collection of transdermal glucose (TG). Due to their comparable skin properties, diffusion studies were performed on full thickness Yucatan miniature pig skin mounted to an in-line diffusion flow cell and on different skin sites of human subjects. Collected TG samples were measured with a L255C mutant of the E. coli glucose-binding protein (GBP) with an attached fluorescent probe. The binding constant (Kd = 0.67 μM) revealed the micromolar sensitivity and high selectivity of the his-tagged GBP biosensor for glucose, making it suitable for TG measurements. In both the animal and human models, skin permeability and TG diffusion across the skin increased with MN application. For intact and MN-treated human skin, a significant positive linear correlation (r > 0.95, p < 0.01) existed between TG and BG. The micromolar sensitivity o...Continue Reading

References

Jan 30, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·F A Quiocho
Aug 4, 1998·Journal of Pharmaceutical Sciences·S HenryM R Prausnitz
Dec 20, 1999·Pharmaceutical Research·V K GuptaM Rerek
Nov 5, 2003·Biotechnology and Bioengineering·Xudong GeGovind Rao
Feb 28, 2004·Analytical Chemistry·Xudong GeGovind Rao
Mar 2, 2005·Diabetes Technology & Therapeutics·Ping M WangMark R Prausnitz
Jul 23, 2005·Archives of Disease in Childhood. Fetal and Neonatal Edition·K BeardsallD B Dunger
May 15, 2008·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Holley F AllenChristine A McKiernan
Sep 17, 2009·Pediatric Diabetes·Joseph WolfsdorfRagnar Hanas
Nov 4, 2009·Journal of Diabetes Science and Technology·Puneet KhannaShekhar Bhansali
Feb 11, 2010·Journal of Diabetes Science and Technology·Jeffrey I JosephZvi Grunwald
Feb 20, 2010·Journal of Diabetes Science and Technology·Christopher G PrettyMatthew Signal
Mar 27, 2010·The Journal of Pediatrics·Deborah L HarrisJane E Harding
Jun 2, 2010·Journal of Diabetes Science and Technology·Matthew SignalGeoffrey M Shaw
Mar 10, 2012·Journal of Diabetes Science and Technology·Kupper A WintergerstCharles R Woods
Dec 25, 2013·Therapeutic Drug Monitoring·Ryan F DonnellyJames C McElnay
May 31, 2014·Journal of Diabetes Science and Technology·Sunsanee KanjananimmanontLeah Tolosa
May 31, 2014·Journal of Diabetes Science and Technology·Arvind JinaMark Christiansen
Nov 5, 2014·Analytical Chemistry·Andrey V RomanyukMark R Prausnitz
Apr 14, 2015·Experimental Dermatology·Maelíosa T C McCruddenRyan F Donnelly
Oct 17, 2016·Analytical and Bioanalytical Chemistry·Sanjiv SharmaAnthony E G Cass
Mar 12, 2017·Analytical and Bioanalytical Chemistry·Cristina TiangcoLeah Tolosa
May 28, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shubhmita BhatnagarVenkata Vamsi Krishna Venuganti

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Citations

Aug 30, 2021·Current Opinion in Biotechnology·Kenta IitaniGovind Rao

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