Minimally invasive transabdominal collection of preimplantation embryos from the common marmoset monkey (Callithrix jacchus)

Theriogenology
Kisaburo HanazawaE Sasaki

Abstract

A novel, minimally invasive, transabdominal embryo collection method (transabdominal method) was developed as an alternative to a standard abdominal incision for embryo collection in the common marmoset. The abdominal incision method was used for 304 flushes using 36 female animals, whereas the transabdominal method was used for 488 flushes using 48 females; successful embryo collection rates were 48.0% and 48.4% (P > 0.05), respectively. These techniques were successfully duplicated at another institute (German Primate Center, DPZ). At that institution, successful embryo collection rates were 88.9% and 77.8% for the abdominal incision and transabdominal methods, respectively (P > 0.05), whereas the average numbers of preimplantation embryos obtained per flush were (mean ± SD) 1.91 ± 0.35 and 1.71 ± 0.14 (P > 0.05). The transabdominal method reduced animal stress, did not require incisional wound healing, and enabled successive embryo recoveries to be done much sooner. More embryos in early developmental stages (zygotes/morulae) were recovered using the transabdominal method (76.1%) than the abdominal incision method (52.6%, P < 0.01). In contrast, recovery of arrested or abnormal embryos was not significantly different between...Continue Reading

References

Jan 1, 1985·Journal of Reproduction and Fertility·P M SummersJ K Hodges
Aug 1, 1994·Journal of Medical Primatology·J A ThomsonJ P Hearn
Sep 1, 1993·Journal of Reproduction and Fertility·M HeistermannJ K Hodges

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Citations

May 21, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·B FereydouniR Behr
May 31, 2013·Reproductive Biology·Hidetoshi IshibashiKazuhiko Seki
Mar 9, 2019·International Journal of Molecular Sciences·Yoon Young KimSeung-Yup Ku

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