Minimally processed fresh frozen human reference sera: preparation, testing, and application to international external quality assurance

Scandinavian Journal of Clinical and Laboratory Investigation
G M HenriksenA Uldall

Abstract

The preparation of unmodified or minimally processed fresh frozen human sera is described, as well as the previous use of such sera, e.g. in Nordic and international external quality assurance (EQA) activities. The unmodified serum is prepared from fresh donors' blood collected in dry bags and allowed to coagulate. The serum is collected "on the clot", pooled, filtered, mixed, dispensed in polypropylene vials and frozen at -80 degrees C without further processing. Some batches were slightly modified by spiking or dilution. Critical steps of the production and use of the sera are described and improvements are discussed. A total of 34 different batches have been prepared since 1985. Results from homogeneity and stability studies are presented. The studies cover 18 routine components in serum stored at +4 degrees C to 37 degrees C for up to 34 days. Good stability was observed for storage of all components, with the exception of triglyceride. Amylase, creatininium, glucose, gamma-glutamyltransferase, urate (and perhaps carbamide) showed deterioration after 13 days of incubation at 37 degrees C. The long-term stability at -80 degrees C is reviewed and new data are presented, e.g. as consensus values from EQA schemes, where the sam...Continue Reading

References

Feb 1, 1989·Scandinavian Journal of Clinical and Laboratory Investigation·A UldallS Bak
Oct 1, 1985·Clinical Biochemistry·G Letellier, F Desjarlais
May 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·A Uldall

❮ Previous
Next ❯

Citations

Jun 6, 2017·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sunyoung AhnHyon-Suk Kim
Jan 16, 2014·Indian Journal of Clinical Biochemistry : IJCB·Rixin Jamtsho
Jan 15, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Peter RidefeltAnders Larsson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists
Hiroaki IkesueJill M Kolesar
© 2022 Meta ULC. All rights reserved