Abstract
Phosphatidylserine (PS) is useful as the additive in industries for memory improvement, mood enhancement and drug delivery. Conventionally, PS was extracted from soybeans, vegetable oils, egg yolk, and biomass; however, their low availability and high extraction cost were limiting factors. Phospholipase D (PLD) is a promising tool for enzymatic synthesis of PS due to its transphosphatidylation activity. In this contribution, a new and uncharacterized PLD was first obtained from GenBank database via genome mining strategy. The open reading frame consisted of 1614 bp and potentially encoded a protein of 538-amino-acid with a theoretical molecular mass of 60 kDa. The gene was successfully cloned and expressed in Escherichia coli. Its enzymatic properties were experimentally characterized. The temperature and pH optima of PLD were determined to be 60°C and 7.5, respectively. Its hydrolytic activity was improved by addition of Ca2+ at 5 mM as compared with the control. The enzyme displayed suitable transphosphatidylation activity and PS could be synthesized with L-serine and soybean lecithin as substrates under the catalysis of PLD. This PLD enzyme might be a potential candidate for industrial applications in PS production. To the b...Continue Reading
References
Jan 1, 1979·Journal of Biochemistry·S Imamura, Y Horiuti
Mar 1, 1990·Acta Psychiatrica Scandinavica·M MaggioniF Brambilla
May 1, 1991·Neurology·T H CrookD C Massari
May 1, 1987·Epilepsy Research·C LoebP Scotto
Jun 15, 1988·Biochimica Et Biophysica Acta·L R JunejaS Shimizu
Feb 1, 1986·Acta Neurologica Scandinavica·P J DelwaideM Ylieff
Nov 11, 1994·Nucleic Acids Research·J D ThompsonT J Gibson
Oct 1, 1993·Cellular and Molecular Neurobiology·M V RapallinoH Hydén
May 1, 1996·Trends in Pharmacological Sciences·A J MorrisM A Frohman
Sep 16, 1996·FEBS Letters·J A MayrF Paltauf
Jan 1, 1996·Neuropsychobiology·F BrambillaT Cenacchi
Feb 17, 1999·Journal of Biochemistry·C OginoH Fukuda
Mar 17, 1999·Analytical Biochemistry·I AurichR Ulbrich-Hofmann
Apr 25, 2000·Progress in Lipid Research·X Wang
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·H InterthalJ J Champoux
May 4, 2002·Reviews of Physiology, Biochemistry and Pharmacology·J H Exton
Oct 29, 2002·FEBS Letters·John H Exton
Jan 24, 2004·Applied Microbiology and Biotechnology·C OginoH Fukuda
Mar 15, 2005·Biotechnology Advances·Zheng GuoXuebing Xu
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Oct 3, 2007·Journal of the International Society of Sports Nutrition·Ralf JägerMichael Kingsley
Nov 28, 2007·Archives of Pharmacal Research·Jaya Ram SimkhadaJin Cheol Yoo
Feb 21, 2009·The Journal of Microbiology·Ji-Seon LeeDoo Hyun Nam
Sep 11, 2012·Biochimica Et Biophysica Acta·Jean E Vance, Guergana Tasseva
May 21, 2014·Journal of Agricultural and Food Chemistry·Yihan LiuFuping Lu
Jun 10, 2015·Phytochemistry·Hafeeza KhatoonRenate Ulbrich-Hofmann
Citations
Apr 17, 2019·Bioprocess and Biosystems Engineering·Hai-Juan HouJin-Song Shi
Apr 4, 2018·The Journal of Microbiology·Yu ZhaoChunzhi Zhang
Feb 8, 2019·Applied Microbiology and Biotechnology·Sebastián CerminatiMaría Eugenia Castelli
Jan 5, 2020·Applied Microbiology and Biotechnology·Israel García-CanoRafael Jiménez-Flores
Nov 5, 2020·Biomolecules·Vicneswarry DorairajNor Azian Abdul Murad
Mar 20, 2021·Colloids and Surfaces. B, Biointerfaces·Yao ZhangHui Zhang
May 15, 2021·Critical Reviews in Biotechnology·Peng ZhangJin-Song Shi