Mining the mouse transcriptome of receptive endometrium reveals distinct molecular signatures for the luminal and glandular epithelium

Endocrinology
Andrea L Niklaus, Jeffrey W Pollard

Abstract

Epithelia coat most tissues where they sense and respond to the environment and participate in innate immune responses. In the adult mouse uterus, columnar epithelium lines the central lumen and the glands that penetrate the underlying stroma. A nidatory surge of estrogen causes differentiation of the luminal epithelium to the receptive state that permits blastocyst attachment and allows subsequent implantation. Here, using laser-capture microdissection to isolate the luminal and glandular epithelia separately, we have profiled gene expression 2 h before embryo attachment to determine whether there are unique roles for these two epithelial structures in this process. Although most genes were expressed in both compartments, there was greater expression of 153 and 118 genes in the lumen and glands, respectively. In the luminal epithelium, there is enrichment in lipid, metal-ion binding, and carbohydrate-metabolizing enzymes, whereas in the glands, immune response genes are emphasized. In situ hybridization to uterine sections obtained from mice during the preimplantation period validated these data and indicated an array of previously undocumented genes expressed with unique patterns in these epithelia. The data show that each ep...Continue Reading

References

Oct 1, 1991·Current Opinion in Immunology·J W Pollard
Jan 1, 1991·The International Journal of Biochemistry·K StaceyL Martin
Jun 1, 1989·Baillière's Clinical Obstetrics and Gynaecology·S C Bell, J O Drife
Jun 15, 1982·European Journal of Biochemistry·L G Hennighausen, A E Sippel
Sep 1, 1981·Journal of Reproduction and Fertility·O BernardD Bennett
Aug 1, 1995·Endocrinology·G A SurveyorD D Carson
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·A J da SilvaC E Rudd
Jun 12, 1998·Biology of Reproduction·J W PollardL Zhu
Jul 2, 1998·Biochemical and Biophysical Research Communications·Y SakakibaraM C Liu
Aug 12, 1998·The Journal of Investigative Dermatology·Y N KaliaR H Guy
Jun 2, 2000·Biochemical and Biophysical Research Communications·K YoshiokaS Sakai
Jun 30, 2000·Seminars in Cell & Developmental Biology·J S HuntT G Burnett
Jul 7, 2000·Developmental Biology·D D CarsonK Yoshinaga
Sep 28, 2000·Reviews of Reproduction·S A Robertson
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
May 23, 2001·Biology of Reproduction·C A GrayT E Spencer
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·J G ChengC L Stewart
Oct 24, 2001·Biology of Reproduction·C A GrayT E Spencer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alain P GobertKeith T Wilson
Jun 6, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hajime WatanabeTaisen Iguchi
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Roman AizmanHaim Garty
Sep 28, 2002·Molecular Endocrinology·Norio TakamotoFrancesco J DeMayo
Jan 17, 2003·Molecular Human Reproduction·Jane M BorthwickStephen K Smith
May 24, 2003·Annals of the New York Academy of Sciences·Kathleen J SweadnerRandall K Wetzel
Jul 31, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Y F TanY L Wang

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Citations

Oct 29, 2013·Reproductive Biomedicine Online·Charlotte H E WeimarNick S Macklon
Feb 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Thomas E SpencerEckhard Wolf
Jul 29, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Hyo Won AhnThomas E Spencer
Sep 24, 2013·Reproductive Biomedicine Online·Charlotte H E WeimarNick S Macklon
Dec 16, 2006·Reproductive Biomedicine Online·J D Aplin
Dec 15, 2015·Differentiation; Research in Biological Diversity·Gracy Xavier RosarioColin L Stewart
May 30, 2015·Journal of Animal Science and Biotechnology·Haichao ZhaoJianhui Tian
May 29, 2012·Biochimica Et Biophysica Acta·Maria Ruiz-AlonsoCarlos Simón
Feb 15, 2013·Biology of Reproduction·Justyna Filant, Thomas E Spencer
Dec 4, 2015·Biology of Reproduction·Kathleen M RahmanCarol A Bagnell
Sep 13, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Justyna FilantThomas E Spencer
Sep 9, 2006·Human Reproduction Update·J A HorcajadasC Simón
Sep 27, 2018·Reproduction, Fertility, and Development·Iside ScaravaggiStefan Bauersachs
May 11, 2019·Endocrine Reviews·Andrew M KelleherThomas E Spencer
Jul 19, 2019·Biology of Reproduction·Vera A van der WeijdenSusanne E Ulbrich
Aug 29, 2021·Proceedings of the National Academy of Sciences of the United States of America·Vera A van der WeijdenSusanne E Ulbrich

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