Mining the secretome of the root-knot nematode Meloidogyne chitwoodi for candidate parasitism genes.

Molecular Plant Pathology
Erwin RozeG Smant

Abstract

Parasite proteins secreted at the interface of nematode and host are believed to play an essential role in parasitism. Here, we present an efficient pipeline of bio-informatic algorithms and laboratory experiments to identify candidate parasitism genes within nematode secretomes, i.e. the repertoire of secreted proteins in an organism. We performed our approach on 12 218 expressed sequence tags (ESTs) originating from three life stages of the plant parasitic nematode Meloidogyne chitwoodi--a molecularly unexplored root-knot nematode species. The ESTs from M. chitwoodi were assembled into 5880 contigs and open reading frames translated from the consensus sequences were searched for features of putative signal peptides for protein secretion and trans-membrane regions, resulting in the identification of 398 secretome members. The products of parasitism genes are secreted by a range of organs, including the oesophageal, amphidial and rectal glands, the intestine, and the hypodermis. To localize the site of expression in M. chitwoodi, we subjected the most abundant secretome members to in situ hybridization microscopy. We found hybridization of one tag in the dorsal oesophageal gland, seven in the two subventral oesophageal glands, ...Continue Reading

References

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Citations

Feb 3, 2009·BMC Genomics·Axel A EllingThomas J Baum
Aug 19, 2014·BioMed Research International·Dana DitgenEva Liebau
Mar 30, 2010·Molecular and Biochemical Parasitology·Martin J WubbenBrian S Scheffler
Oct 20, 2017·Frontiers in Plant Science·Muhammad A AliHolger Bohlmann
Jun 6, 2018·International Journal of Molecular Sciences·Muhammad Amjad AliGyuhwa Chung
Jun 20, 2008·The New Phytologist·Victoria L FullerPeter E Urwin

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