Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides

Bioinformatics
João Miguel FreireMiguel A R B Castanho

Abstract

The need for more effective and safer pharmaceuticals is a persistent quest. Microbial adaptations create the need to permanently develop new antimicrobials (AMPs), for instance. Similarly, intracellular delivery of drugs is still a challenge and translocation of membranes for drug delivery is an area of intense research. Peptides can be used both as AMP drug leads and drug carrier systems for intracellular delivery. Multifunctional proteins are abundant in viruses but, surprisingly, have never been thoroughly screened for bioactive peptide sequences. Using the AMPA and CellPPD online tools, we have evaluated the propensity of viral proteins to comprise AMP or cell-penetrating peptides (CPPs). Capsid proteins from both enveloped and non-enveloped viruses, and membrane and envelope proteins from enveloped viruses, in a total of 272 proteins from 133 viruses, were screened to detect the presence of potential AMP and CPP sequences. A pool of 2444 and 426 CPP and AMP sequences, respectively, were discovered. The capsids of flaviviruses are the best sources of these peptides reaching more than 80% of CPP sequence coverage per protein. Selected sequences were tested experimentally and validated the results. Overall, this study reveal...Continue Reading

References

Dec 10, 1998·Nucleic Acids Research·A BatemanE L Sonnhammer
Apr 26, 2000·Genome Research·C A Ouzounis, P D Karp
Oct 24, 2001·The Journal of Biological Chemistry·Johannes P M Langedijk
Jan 25, 2002·Nature·Michael Zasloff
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lixin MaCarol Beth Post
Jun 24, 2004·Molecular Diversity·J P M LangedijkR H Meloen
Jun 25, 2004·Nucleic Acids Research·Karsten HillerDieter Jahn
May 13, 2006·Science·Trevor Douglas, Mark Young
Nov 16, 2007·Nucleic Acids Research·Nicolas HuloChristian J A Sigrist
Feb 17, 2009·Nature Reviews. Microbiology·Manuel N MeloMiguel A R B Castanho
Aug 27, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ikuhiko NakaseShiroh Futaki
Oct 22, 2009·Nucleic Acids Research·UNKNOWN UniProt Consortium
May 14, 2010·Nucleic Acids Research·Anne de JongOscar P Kuipers
Jun 23, 2010·The Journal of Biological Chemistry·Carla S AlvesMiguel A R B Castanho
Oct 16, 2010·Nucleic Acids Research·Chantal HuloPhilippe Le Mercier
Nov 27, 2010·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Feb 22, 2011·European Biophysics Journal : EBJ·Katrin Splith, Ines Neundorf
Apr 15, 2011·Current Opinion in Biotechnology·Arjen van den Berg, Steven F Dowdy
Jun 15, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Annarita FalangaStefania Galdiero
Nov 5, 2011·Bioinformatics·Marc TorrentDavid Andreu
Mar 10, 2012·Database : the Journal of Biological Databases and Curation·Ankur GautamG P S Raghava
Apr 3, 2012·Drug Discovery Today·Francesca Milletti
Apr 10, 2012·Annual Review of Biochemistry·Nicola G A AbresciaDavid I Stuart
Jun 12, 2012·Trends in Molecular Medicine·Erez Koren, Vladimir P Torchilin
Aug 28, 2012·Current Gene Therapy·Maliha Zahid, Paul D Robbins
Dec 21, 2012·Chemical Biology & Drug Design·David J CraikDavid Price
Jan 29, 2013·Current Opinion in Virology·Theodore C Pierson, Margaret Kielian
Mar 23, 2013·Journal of Translational Medicine·Ankur GautamGajendra P S Raghava
Apr 17, 2013·Scientific Reports·Kristopher MontroseGeoffrey W Krissansen
May 10, 2013·Nature Biotechnology·Jeffrey L Fox
Jun 4, 2013·Drug Discovery Today·Allan A Kaspar, Janice M Reichert
Jun 14, 2013·Virology Journal·Jose A Usme-CiroJuan C Gallego-Gomez
Jul 31, 2013·Current Opinion in Virology·J Cesar Ignacio-EspinozaMatthew B Sullivan
Aug 10, 2013·Current Opinion in Microbiology·Carlos F Arias
Nov 23, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Marco M DominguesNuno C Santos
Nov 23, 2013·Nucleic Acids Research·Faiza Hanif WaghuSusan Idicula-Thomas
Dec 18, 2013·PloS One·João Miguel FreireMiguel A R B Castanho
Feb 25, 2014·ACS Nano·Dana Maria CopoloviciÜlo Langel
Mar 25, 2014·IUBMB Life·Inês Rego de FigueiredoMiguel A R B Castanho

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Citations

Mar 10, 2016·Journal of Chemical Information and Modeling·Alejandro Speck-PlancheM N D S Cordeiro
Nov 4, 2015·IEEE/ACM Transactions on Computational Biology and Bioinformatics·Jingsong ZhangYongyong Shi
Dec 15, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Shicai LiuHeng Zheng
Jan 18, 2017·Antimicrobial Agents and Chemotherapy·Marta GomarascaChristian Rüter
Feb 14, 2020·Current Pharmaceutical Design·Marco Calvinho CavacoVera Luísa Santos Neves
May 20, 2017·Frontiers in Microbiology·Susana A DiasAna S Veiga
Oct 22, 2016·BMC Genomics·Najla KharratAhmed Rebai
Jun 6, 2019·Pharmaceuticals·Boris VishnepolskyMalak Pirtskhalava
May 28, 2019·The Journal of Antimicrobial Chemotherapy·Sandra N PintoAna Salomé Veiga
May 10, 2020·Biomolecules·Szilvia BőszeKatalin Uray
Oct 21, 2020·Current Pharmaceutical Design·Mallikarjuna KoriviBetty R Liu

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