MinION rapid sequencing: Review of potential applications in neurosurgery

Surgical Neurology International
Arpan PatelMark C Preul

Abstract

Gene sequencing has played an integral role in the advancement and understanding of disease pathology and treatment. Although historically expensive and time consuming, new sequencing technologies improve our capability to obtain the genetic information in an accurate and timely manner. Within neurosurgery, gene sequencing is routinely used in the diagnosis and treatment of neurosurgical diseases, primarily for brain tumors. This paper reviews nanopore sequencing, an innovation utilized by MinION and outlines its potential use for neurosurgery. A literature search was conducted for publications containing the keywords of Oxford MinION, nanopore sequencing, brain tumor, glioma, whole genome sequencing (WGS), epigenomics, molecular neuropathology, and next-generation sequencing (NGS). In total, 64 articles were selected and used for this review. The Oxford MinION nanopore sequencing technology has had successful applications within clinical microbiology, human genome sequencing, and cancer genotyping across multiple specialties. Technical details, methodology, and current use of MinION sequencing are discussed through the prism of potential applications to solve neurosurgery-related scientific and diagnostic questions. The MinION...Continue Reading

References

Jun 18, 2015·Clinical Genetics·K D ChristensenUNKNOWN MedSeq Project Team
Aug 1, 2015·International Journal of Legal Medicine·Eva-Lena StattinAase Wisten
Feb 24, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Preety GhanghasS N Sanyal
Apr 15, 2017·Journal of Neurosurgery·Aril Løge HåvikMorten Lund-Johansen
Oct 12, 2017·Nature·Jay ShendureRobert H Waterston

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Citations

Apr 12, 2019·Briefings in Bioinformatics·Saman ZeeshanZeeshan Ahmed
May 1, 2019·Development, Growth & Differentiation·Nobuaki Kono, Kazuharu Arakawa
Jun 26, 2020·Frontiers in Microbiology·D'Arcy R Meyer-DombardJudy Malas
Oct 31, 2020·Sensors·Andrew BogardDaniel Fologea

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Methods Mentioned

BETA
biopsy
chemical treatment
amplicon sequencing
nucleic acid sequencing

Software Mentioned

LAST
- Aligner ( BWA )
Falcon
Burrows
BLAST
MinKNOW
MinION
Galaxy
Scrappie
PacBio Corrected Reads ( PBcR ) Assembler

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