Minocycline block copolymer micelles and their anti-inflammatory effects on microglia

Macromolecular Bioscience
Ghareb Mohamed SolimanFrançoise M Winnik

Abstract

MH, a semisynthetic tetracycline antibiotic with promising neuroprotective properties, was encapsulated into PIC micelles of CMD-PEG as a potential new formulation of MH for the treatment of neuroinflammatory diseases. PIC micelles were prepared by mixing solutions of a Ca(2+)/MH chelate and CMD-PEG copolymer in a Tris-HCl buffer. Light scattering and (1)H NMR studies confirmed that Ca(2+)/MH/CMD-PEG core-corona micelles form at charge neutrality having a hydrodynamic radius approximately 100 nm and incorporating approximately 50 wt.-% MH. MH entrapment in the micelles core sustained its release for up to 24 h under physiological conditions. The micelles protected the drug against degradation in aqueous solutions at room temperature and at 37 degrees C in the presence of FBS. The micelles were stable in aqueous solution for up to one month, after freeze drying and in the presence of FBS and BSA. CMD-PEG copolymers did not induce cytotoxicity in human hepatocytes and murine microglia (N9) in concentrations as high as 15 mg x mL(-1) after incubation for 24 h. MH micelles were able to reduce the inflammation in murine microglia (N9) activated by LPS. These results strongly suggest that MH PIC micelles can be useful in the treatmen...Continue Reading

References

Jul 1, 1995·The Medical Clinics of North America·N C Klein, B A Cunha
Jan 1, 1997·Journal of Esthetic Dentistry·W H Bowles, T J Bokmeyer
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J YrjänheikkiJ Koistinaho
Mar 16, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·V OrtiF Bressolle
Dec 2, 2004·Neurobiology of Disease·David BlumMarc Levivier
Mar 15, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jubo LiuChristine Allen
Nov 24, 2005·International Journal of Pharmaceutics·Donald E Owens, Nicholas A Peppas
Dec 24, 2005·Expert Opinion on Drug Delivery·Hamidreza Montazeri Aliabadi, Afsaneh Lavasanifar
Jan 28, 2006·International Journal of Pharmaceutics·Maurizio RicciCarlo Rossi
Feb 25, 2006·Stroke; a Journal of Cerebral Circulation·Midori A YenariRona G Giffard
May 17, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Woo-Dong JangKazunori Kataoka
Jul 4, 2006·Pharmacology & Therapeutics·Nobuhiro Nishiyama, Kazunori Kataoka
Dec 21, 2006·Nature Reviews. Neuroscience·Michelle L BlockJau-Shyong Hong
Apr 19, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Antonio Di StefanoFrancesco Barbato
Jul 19, 2008·Macromolecular Bioscience·Marina Talelli, Stergios Pispas
Nov 4, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Vladimir Torchilin
Nov 4, 2008·Behavioural Brain Research·Hye-Sun Kim, Yoo-Hun Suh
Dec 25, 2008·Journal of Biomaterials Science. Polymer Edition·Xiaofei LiangJin Chang
Jan 8, 2009·PloS One·Wei HuOlaf Stüve
Apr 15, 2009·Journal of the Royal Society, Interface·Kensuke OsadaKazunori Kataoka
Apr 18, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Caroline De VochtJan Steyaert

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Citations

Jan 17, 2016·International Journal of Pharmaceutics·Alexander Dontsios HolmkvistJens Schouenborg
Dec 22, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fan ZhangRangaramanujam M Kannan
Oct 16, 2013·Alcoholism, Clinical and Experimental Research·Rajiv G AgrawalSusan E Bergeson
Aug 25, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Pallavi MadhusudananSahadev A Shankarappa
Jun 9, 2015·Journal of Neural Engineering·Zhiling ZhangYinghui Zhong
Mar 6, 2021·Materials Today. Bio·D SilvaA J Salgado
Aug 3, 2020·Advances in Colloid and Interface Science·Ayman El JundiBenjamin Nottelet

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