Minocycline protects against myocardial ischemia/reperfusion injury in rats by upregulating MCPIP1 to inhibit NF-κB activation

Acta Pharmacologica Sinica
Quan YiJian-Dong Luo

Abstract

Minocycline is a tetracycline antibiotic and has been shown to play a protective role in cerebral and myocardial ischemia/reperfusion (I/R). However, the underlying mechanism remains unclear. Herein, we investigated whether monocyte chemotactic protein-induced protein-1 (MCPIP1), a negative regulator of inflammation, was involved in the minocycline-induced cardioprotection in myocardial I/R in vivo and in vitro models. Myocardial ischemia was induced in rats by left anterior descending coronary artery occlusion for 1 h and followed by 48 h reperfusion. Minocycline was administered prior to ischemia (45 mg/kg, ip, BID, for 1 d) and over the course of reperfusion (22.5 mg/kg, ip, BID, for 2 d). Cardiac function and infarct sizes were assessed. Administration of minocycline significantly decreased the infarct size, alleviated myocardial cell damage, elevated left ventricle ejection fraction, and left ventricle fractional shortening following I/R injury along with significantly decreased pro-inflammatory cytokine IL-1β and monocyte chemoattractant protein-1 (MCP-1) levels in heart tissue. H9c2 cardiomyocytes were subjected to oxygen glucose deprivation (OGD) followed by reoxygenation (OGD/R). Pretreatment with minocycline (1-50 μmo...Continue Reading

References

Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J YrjänheikkiJ Koistinaho
Jan 3, 2001·The Journal of Clinical Investigation·P P Tak, G S Firestein
Feb 8, 2006·Annual Review of Physiology·Viviany R TaquetiAndrew H Lichtman
Feb 25, 2006·Cardiovascular Research·Fabio Di Lisa, Paolo Bernardi
Jan 8, 2008·The Journal of Biological Chemistry·Jian LiangMingui Fu
Jan 1, 1997·Mediators of Inflammation·H S Sharma, D K Das
Oct 14, 2008·Journal of the American College of Cardiology·Diego Romero-PerezFrancisco Villarreal
Sep 15, 2009·The FEBS Journal·Lukasz SkalniakJolanta Jura
Oct 13, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Steffen BohlCraig A Lygate
May 12, 2010·Cold Spring Harbor Perspectives in Biology·Toby Lawrence
Dec 1, 2010·The Journal of Experimental Medicine·Jian LiangMingui Fu
Jan 5, 2011·Antioxidants & Redox Signaling·Regina Brigelius-Flohé, Leopold Flohé
Jan 27, 2011·Anatomy & Cell Biology·Wendy C HuangMidori A Yenari
Sep 13, 2011·Translational Stroke Research·Susan C FaganDavid C Hess
Jan 10, 2012·Circulation Research·David J MarchantBruce M McManus
Jan 31, 2012·Transplant Immunology·Sabarinathan RamachandranWilliam C Chapman
Oct 31, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Katsunori TaiTakanori Ueda
Feb 28, 2013·British Journal of Pharmacology·N Garrido-MesaJ Gálvez
Apr 11, 2015·Journal of the American College of Cardiology·Borja IbáñezFrans Van de Werf
Aug 9, 2016·Annals of Translational Medicine·Fabian Sanchis-GomarAlejandro Lucia
Dec 19, 2016·Nature Reviews. Immunology·Mingui Fu, Perry J Blackshear
Feb 13, 2018·Cardiovascular Drugs and Therapy·Kuixiang ChenYinghua Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
Assay
transfection
X-ray
nuclear translocation
transfections

Software Mentioned

SPSS17
Quantity One
Image Pro Plus

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.