Mint3-mediated L1CAM expression in fibroblasts promotes cancer cell proliferation via integrin α5β1 and tumour growth

Oncogenesis
Hiroki J NakaokaTakeharu Sakamoto

Abstract

Fibroblasts are some of the major cells in tumour tissues that influence tumour progression and drug resistance. However, our understanding on fibroblast-mediated tumour malignancy remains incomplete. Munc18-1-interacting protein 3 (Mint3) is known as an activator of hypoxia-inducible factor-1 (HIF-1) even during normoxia in cancer cells, macrophages and fibroblasts. Although Mint3 promotes ATP production via glycolysis by activating HIF-1 in cancer cells and macrophages, the biological role of Mint3-mediated HIF-1 activation in fibroblasts remains unclear. To address this, we examined whether Mint3 in fibroblasts contributes to tumour growth. Mint3 depletion in mouse embryonic fibroblasts (MEFs) decreased tumour growth of co-injected human breast cancer cells, MDA-MB-231 and epidermoid carcinoma A431 cells in mice. In MEFs, Mint3 also promoted cancer cell proliferation in vitro in a cell-cell contact-dependent manner. Mint3-mediated cancer cell proliferation depended on HIF-1, and further gene expression analysis revealed that the cell adhesion molecule, L1 cell adhesion molecule (L1CAM), was induced by Mint3 and HIF-1 in fibroblasts. Mint3-mediated L1CAM expression in fibroblasts stimulated the ERK signalling pathway via inte...Continue Reading

References

Dec 22, 1998·European Journal of Cell Biology·M Okamoto, T C Südhof
Mar 12, 2003·Cell·Thorsten CramerRandall S Johnson
May 28, 2005·Journal of Cellular Physiology·Yoshifumi Itoh, Motoharu Seiki
Sep 21, 2006·Molecular Cancer Therapeutics·Parisa KhaliliShafaat A Rabbani
Apr 29, 2008·Nature Medicine·Greg FinakMorag Park
May 24, 2008·Molecular Cell·William G Kaelin, Peter J Ratcliffe
Apr 18, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takeharu Sakamoto, Motoharu Seiki
Nov 28, 2009·Current Opinion in Genetics & Development·Gregg L Semenza
Oct 23, 2010·Molecular Cell·Amar J MajmundarM Celeste Simon
Nov 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yasushi KojimaAkira Orimo
Jan 5, 2011·Trends in Molecular Medicine·Helena KiefelPeter Altevogt
Mar 5, 2011·The Journal of Biological Chemistry·Takeharu SakamotoMotoharu Seiki
Sep 7, 2011·Biochemical and Biophysical Research Communications·Toshiro HaraTakeharu Sakamoto
Dec 16, 2011·Nature Reviews. Cancer·Brian KeithM Celeste Simon
Feb 7, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Josephine HaiMing-Sound Tsao
Feb 22, 2012·The Journal of Cell Biology·Pengfei LuZena Werb
Mar 23, 2012·Immunological Reviews·Joseph A DiDonatoMichael Karin
Jul 17, 2012·Cell Adhesion & Migration·Helena KiefelPeter Altevogt
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Weibo LuoGregg L Semenza
Mar 6, 2013·Journal of Cellular Physiology·Urszula M Polanska, Akira Orimo
May 21, 2013·Oncogene·K IeguchiY Maru
Oct 30, 2013·Molecular and Cellular Biology·Takeharu SakamotoMotoharu Seiki
Nov 10, 2013·Nature Medicine·Daniela F Quail, Johanna A Joyce
Jul 30, 2014·The Journal of Experimental Medicine·Daniel ÖhlundDavid Tuveson
Aug 19, 2015·Advanced Drug Delivery Reviews·Genichiro IshiiShinya Neri
May 7, 2016·Genes & Development·Philippe Gascard, Thea D Tlsty

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Citations

Jun 22, 2017·Biochemical and Biophysical Research Communications·Toshiro HaraTakeharu Sakamoto
Nov 4, 2017·International Journal of Molecular Sciences·Andrea NicoliniAngelo Carpi
May 23, 2019·Current Opinion in Clinical Nutrition and Metabolic Care·Andrew RedfernErik W Thompson
Aug 29, 2019·International Journal of Molecular Sciences·Miriam van der MatenMirjam M Zegers
Feb 13, 2020·Frontiers in Genetics·Peiling XieHuimin Zhang
Jan 8, 2021·OncoTargets and Therapy·Jianbing HouHongjuan Cui
Jul 3, 2021·Cancers·Patricia Fernández-NogueiraPaloma Bragado
Jul 13, 2021·Chinese Journal of Natural Medicines·Chao YangYi-Tao Wang
Aug 10, 2021·World Journal of Gastroenterology : WJG·Matias Estaras, Antonio Gonzalez

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Methods Mentioned

BETA
immunoprecipitation
transfection
xenograft
PCR
fluorescence microscopy
Assay
confocal microscopy
surgical resection
FACS

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